Biju Bharali

TL;DR: The present review has summarized the recent developments in the expression of foreign proteins in P. pastoris and discussed various cellular engineering strategies which include codon optimization, pathway engineering, signal peptide processing, development of protease deficient strain and glyco-engineered strains for the high yield protein secretion of recombinant protein.

TL;DR: In this article, a yeast strain was identified as Rhodotorula mucilaginosa by 26s rRNA sequencing and two-stage optimization using taguchi orthogonal array method and multi objective optimization using genetic algorithm was applied for the development of an optimized medium.

TL;DR: It is shown that the batch reactor kinetics with complex medium showed that the product is growth associated and maximum production yield of 1.98 mg L −1 was observed at a 72 hr time interval, and the efficacy of rhIFN-γ on an anti-proliferative property was evaluated.

TL;DR: How resident endophytic fungi aids the host plant in tackling various forms of biotic and abiotic stresses is discussed and data reservoir on fungal endophytes has been constantly growing over the past decades and this reservoir can pave the route of future exploration of these beneficial organisms in therapeutics, biology and evolution via emergent and evolving modes of analysis like machine learning and more.

TL;DR: The applications of ENM in plant biology with respect to nutrient management, regulation of plant growth, gene detection and preservation, disease diagnostics and control, and the techniques for enhanced analyses and controlled delivery of functional/bioactive molecules and decreased cytotoxicity are discussed.