Showing papers by "Biswanath Bhunia published in 2018"
TL;DR: This review explains how different natural circumstances have attributed to the advancement of EPS production, thereby increasing the capacity of bioremediation to deal the issue of heavy metal contamination in both soil and water.
111 citations
TL;DR: This review covers genetic regulation for production of exopolysaccharide, analytical strategies for their characterization, evaluation of structure property relationship and design of extraction protocol from cyanobacterial biomass for removal of heavy metal from wastewater.
80 citations
24 Jul 2018
TL;DR: It is evident that improved production of xylanase can be possible through optimization of environmental stresses during spore preservation, seed cultivation and batch fermentation and can be intensified through identification of markers at various stages of fermentation process.
Abstract: Improved xylanase production was carried out through optimization of environmental stresses during spore preservation, seed cultivation and batch fermentation and identifies the markers at various stages. The maximum spore size (radius 6.5 µm) of Aspergillus niger was noticed after 28 days of spore preservation. During seed cultivation, the hypha formed alongside of germination tube (length 196.8 µm) was noticed only at pH-7 after 18 h of incubation at 28 °C. Therefore, pH-7 and 28 °C were considered as optimum during seed cultivation. In this stage, the final pH of the medium was found to be 6.2 which can be used as marker for completion of seed culture. The production media was optimized through Taguchi methodology. The maximum xylanase production was found to be 1575.93 U. The optimum concentration for media components was found to be xylan from beechwood of 3 g/l, potassium nitrate of 10 g/l, magnesium sulphate of 5 g/l, di-potassium hydrogen phosphate of 50 mM, calcium carbonate of 2 g/l, 1000× of trace element (1 ml) and sodium chloride of 5 g/l. It is evident that improved production of xylanase can be possible through optimization of environmental stresses during spore preservation, seed cultivation and batch fermentation and can be intensified through identification of markers at various stages of fermentation process.
3 citations
01 Oct 2018
TL;DR: In this paper, the maximum reducing sugar per gram of orange peel was found to be 315.54 mg/g in presence of 2.5% (v/v) H 2 SO 4 at 15 psi for 30 min reaction time.
Abstract: In the present study, the orange peels were hydrolyzed at 121°C, 15 psi for 15 mins, 30mins and 45 mins using sulphuric acid (H 2 SO 4 ) at concentration ranging from 2.5 to 15 %(v/v). Furthermore enzymatic treatment process was carried out by using equal amount of xylanase enzyme to acid hydrolysates after neutralization with sodium hydroxide (NaOH) at a concentration of 5% (w/v). The enzymatic process was carried out at 37°C at 180rpm and reducing sugar was measured after 1, 2 and 3 hr of enzymatic treatment. It was found that the maximum reducing sugar per gram of orange peel was found to be 315.54 mg/g in presence of 2.5% (v/v) H 2 SO 4 at 15 psi for 30 min reaction time which was considered to be optimum. The maximum reducing sugar found during enzymatic treatment was 616.81mg/g at 3rd hour, 310.19mg/g at 2nd hour and 274.50 mg/g at 1st hour respectively from the sample which was hydrolyzed by 2.5% (v/v) sulphuric acid. Since enzymatic conversion rate for production of reducing sugar from acid hydrolysates was found to be higher after one hour enzyme treatment process, therefore, it was considered to be optimum. The reducing sugar production rate was found to be decreasing at various incubation times may be due to inhibition of enzyme. Therefore, the maximum reducing sugar (582.48 mg/gm of orange peel) can be produced by optimization of acid hydrolysis and enzymatic treatment.