The preparation and antihyperlipidaemic assay of piperlonguminine in vivo

Only one kind of synthesized alkaloid, piperlonguminine, was used to understand the interference of the other alkaloids in pharmacokinetic study using HPLC/UV in rat plasma after oral administration. Compared with the previous report, it was clarified that mixed alkaloids such as piperine and the other extract from Piper longum Linn did not interfere with the results.

LC analysis and pharmacokinetic study of synthetic piperlonguminine in rat plasma after oral administration

Objective To observe the effects of piperlonguminine on the blood lipid metabolism and its related genes expression in hyperlipidemia rats. Methods The hyperlipidemia models induced by high-fat feeding were established. The piperlonguminine group was fed continuously with piperlonguminine (2.5, 5, and 10 mg/kg) for four weeks. At the last day, all the rats fasted for at least 12 h and the blood was drawn for the measurement of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoproteinA1 (ApoA1), and apolipoprotein B (ApoB); The mRNA expression of low density lipoprotein cholesterol receptor (LDLR), ApoB, and 3-hydroxy 3-methylglutaryl co-enzyme A reductase (HMG-CoAR) were measured by RT-PCR. Results Compared with that of the high-fat model group, the serum TC, TG, LDL-C, ApoB levels, and the ratio of ApoB/ApoA1 were reduced (P0.05) and the serum HDL-C and ApoA1 were increased in the piperlonguminine group (P0.05). Piperlonguminine could increase hepatic LDLR mRNA levels (P0.05) and reduce ApoB mRNA levels (P0.05). But it had no significant effects on HMG-CoAR mRNA level (P0.05). Conclusion Piperlonguminine has the effect of regulating lipid metabolism. The mechanism is likely related to increasing LDLR mRNA expression and decreasing ApoB mRNA expression.

Borijihan Gereltu

Papers

Effect of piperlonguminine on blood lipid metabolism and its related genes expression in hyperlipidemia rats