In performing phenotypic characterization tests, the organisms used for the inoculation of test media should be from fresh transfers and in good physiological condition. The methods described in this chapter are based primarily on methods developed for the characterization and identification of organisms which have usually been isolated on nutrient-rich media. While zoologists may be content to classify all insects or all vertebrates, prokaryote systematics generally deals with all prokaryotes and in an evolutionary context attempts to extend this study back to the origin of life itself. To test for acetamide hydrolysis, streak the surface of an acetamide agar slant with a sample from a dilute suspension. Incubate for up to 7 days. Care should be taken to ensure that the basal medium to which the carbohydrates are added does not contain fermentable or oxidizable sugars, and control media without any added carbohydrates should always be included during testing of cultures. Methanogenic members of the Archaea also fluoresce under UV light. Some facultative anaerobes, such as Escherichia coli and Staphylococcus aureus, grow aerobically by respiration with oxygen and grow anaerobically by fermentation.

Phenotypic Characterization and the Principles of Comparative Systematics

Identification of Proteolytic Pseudomonads Isolated from Raw Milk

Über die Wirkung von Colchicin und indomethacin auf die leukozytäre Phagozytose von Staphylococcus aureus und E. coli

The invention discloses a bacterial culture solution for detecting pathogenic bacteria in blood The bacterial culture solution comprises nutrient substances required by the growth of bacteria, an anticoagulant and an antagonistic antibiotic reagent according to the mass-concentration ratio being (35-52):(1-10):(5-20) Compared with the traditional culture medium cultured in blood microbial culture tools such as a blood bacterial enrichment broth and a manual dual-way aerobic hemoline, the bacterial culture solution is rapid in bacterial reproduction, so that the blood detection time is shortened; and in addition, metabolic products of the bacteria can react with bicarbonate in the culture solution so as to be converted to form chemical signals identified by an instrument, so that the blood detection accuracy is high Thus, the bacterial culture solution has a wide application prospect in the field of clinical blood detection

Bacterial culture solution for detecting pathogenic bacteria in blood

A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involved the introduction of blood (and bacteria) into the growth medium with the dye in its colorless state. As the bacteria grew, they converted the dye to a visible blue color (formazan) with their reductases. The growth medium served as its own contamination control, since microbial growth and be detected by a color change before it was used for blood culture. The experiments described herein demonstrate that the composition of this medium (with the dye) provides a unique system that is able to make a reliable and rapid detection of both gram-positive and gram-negative microorganisms and yeasts (Candida albicans) commonly associated with bacteremia.

C N Shih

Papers

New blood culture medium.