Showing papers by "Carlo M. Croce published in 1977"
TL;DR: The somatic cell hybrids between mouse myeloma cells and spleen cells derived from a BALB/c mouse immunized with purified influenza virus found to produce large amounts of antibodies specific for the hemagglutinin of the virus.
Abstract: We have produced somatic cell hybrids between mouse myeloma cells and spleen cells derived from a BALB/c mouse immunized with purified influenza virus. The hybrid cells were found to produce large amounts of antibodies specific for the hemagglutinin of the virus and were able to induce tumor formation when injected into BALB/c mice.
151 citations
TL;DR: Mouse-human somatic cell hybrids that lose (segregate) human chromosomes produce only mouse 28S ribosomal RNA even when they retain copies of the human chromosomes that contain the genes for 28S ribsomal RNA.
Abstract: Mouse-human somatic cell hybrids that lose (segregate) human chromosomes produce only mouse 28S ribosomal RNA even when they retain copies of the human chromosomes that contain the genes for 28S ribosomal RNA. In contrast, mouse-human hybrid cells that segregate mouse chromosomes produce only human 28S ribosomal RNA even when they have retained copies of mouse chromosomes that contain the 28S ribosomal RNA genes.
95 citations
TL;DR: It is indicated that SV40 is integrated in only one of the two parental human chromosomes 17.1.1 in GM54VA human cells transformed by simian virus 40.
Abstract: GM54VA human cells transformed by simian virus 40 (SV40) were fused with peritoneal macrophages obtained from three different mouse strains. All 27 hybrid clones studied were positive for SV40 tumor antigen in 100% of their cells and contained human chromosome 17. Human chromosome 17 was the only human chromosome present in five of the hybrid clones. Fusion of GM54VA cells and either thymidine kinase (EC 2.7.1.75)-deficient mouse or Chinese hamster fibroblasts resulted in the growth in hypoxanthine-aminopterin-thymidine medium of hybrid clones positive and negative for SV40 tumor antigen. Counterselection of the hybrid clones positive for tumor antigen in medium containing 5-bromodeoxyuridine resulted in the growth of hybrid cells that were negative for tumor antigen. These experiments indicate that negative for tumor antigen. These experiments indicate that SV40 is integrated in only one of the two parental human chromosomes 17. Because the genome of SV40 has been assigned to human chromosome 7 in two other SV40-transformed human cell lines, at least two different integration sites for SV40 would seem to be present in human cells: one located in human chromosome 7 and the other located in human chromosome 17.
49 citations
TL;DR: A standard G-band karyotype is proposed for the genus Peromyscus and its chromosomes are numbered and arranged according to chromosome number.
Abstract: £A standard G-band karyotype is proposed for the genus Peromyscus . G-banded chromosomes of Peromyscus boylii glasselli (NF = 56), numbered and arranged according
45 citations
TL;DR: Four different human cell lines transformed by simian virus 40 (SV40) were tested for their tumorigenicity in athymic nude mice and Somatic cell hybrids between either LN-SV or GM54VA SV40-transformed human cells and normal mouse peritoneal macrophages, which have retained the human chromosomes carrying the SV40 genome, were found to be much more tumorigenic than the SV 40-transforming human cell parents.
Abstract: Four different human cell lines transformed by simian virus 40 (SV40) were tested for their tumorigenicity in athymic nude mice. Two of these lines, W18Va2 and GM52VA, were found to be tumorigenic when inoculated at a concentration of 1 x 10(7) cells per mouse. The other two cell lines, LN-SV and GM54VA, were found to induce very small tumors only after the injection of approximately 1 x 10(8) cells per mouse. Somatic cell hybrids between either LN-SV or GM54VA SV40-transformed human cells and normal mouse peritoneal macrophages, which have retained the human chromosomes carrying the SV40 genome, were found to be much more tumorigenic than the SV40-transformed human cell parents. These experiments suggest that the genetic background in which the human chromosomes carrying the SV40 genome are present plays a role in the modulation of the expiration of malignancy.
42 citations
TL;DR: The results of this study indicate that the expression of mouse galactokinase and thymidine kinase segregates concordantly, and, therefore, it is inferred that the gene for mouse thymazine kinase is also located on mouse chromosome 11.
Abstract: We have studied the expression of mouse galactokinase in human-mouse somatic cell hybrids segregating mouse chromosomes. Since concordant segregation of the expression of mouse galactokinase and the presence of mouse chromosome 11 were observed in the hybrid clones, we conclude that the gene for mouse galactokinase is located on mouse chromosome 11. We have also investigated the expression of mouse galactokinase in somatic cell hybrids between thymidine kinase-deficient Chinese hamster cells and mouse peritoneal macrophages. The results of this study indicate that the expression of mouse galactokinase and thymidine kinase segregates concordantly, and, therefore, we infer that the gene for mouse thymidine kinase is also located on mouse chromosome 11.
23 citations
Journal Article•
TL;DR: Using a sensitive radioimmunoassay, it was able to identify noncross-reactive cell-surface antigen(s) specifically coded for by either human chromosome 7 or 17, and present in normal, tumor-derived and virus-transformed human cells, but no reactivity against SV40 tumor-specific surface antigen (TSSA) could be detected in the antisera.
Abstract: Somatic cell hybrids between SV40-transformed human cell lines and mouse peritoneal macrophages (MPM) containing either human chromosome 7 or 17 carrying the SV40 genome were injected into mice syngeneic to the mouse parental cells. Since either chromosome 7 or 17 was the only human chromosome present in the hybrids used as immunogens, the humoral immune response to gene products coded for by either chromosome was assayed. Using a sensitive radioimmunoassay, we were able to identify noncross-reactive cell-surface antigen(s) specifically coded for by either human chromosome 7 or 17, and present in normal, tumor-derived and virus-transformed human cells. However, no reactivity against SV40 tumor-specific surface antigen (TSSA) could be detected in the antisera.
12 citations
TL;DR: Somatic cell hybrids between mouse cells and human cells carrying a reciprocal translocation between chromosomes 1 and 7 were tested for the expression of human enolase 1 (ENO1) by starch-g.
Abstract: Somatic cell hybrids between mouse cells and human cells carrying a reciprocal translocation between chromosomes 1 and 7 were tested for the expression of human enolase 1 (ENO1) by starch-g
9 citations
Journal Article•
TL;DR: When nude mice were reconstituted with normal syngeneic T lymphocytes from spleen or thymus source, the humoral immune responsiveness to SV40 T antigen was restored and normal BALB/c mice readily produce antibodies to SV 40 T antigen.
Abstract: Athymic BALB/c nude mice (nu/nu) fail to generate circulating antibodies to simian virus 40 (SV40) tumor (T) antigen when immunized with SV40-transformed mouse cells or with T antigen positive somatic cell hybrids derived from SV40-transformed human and normal mouse parental cells. However, normal BALB/c mice readily produce antibodies to SV40 T antigen. When nude mice were reconstituted with normal syngeneic T lymphocytes from spleen or thymus source, the humoral immune responsiveness to SV40 T antigen was restored.
5 citations