Showing papers by "Chandra Mohini Chaturvedi published in 2019"
TL;DR: It is concluded that paraquat treatment is capable of activating the HPA axis via upregulating transcription and translation of the hypothalamic neuropeptides AVP and CRH as well as serum corticosterone level.
9 citations
TL;DR: It can be concluded that 12‐hr quail showed significantly increased testicular activity and steroidogenesis while opposite pattern was observed in 8‐hour quail.
Abstract: Specific phase relation of serotonin and dopamine modulate the hypothalamo-hypophyseal-gonadal axis as well as photosexual responses in Japanese quail, but the effect of these specific phase relations on testicular activity and steroidogenesis is not yet been investigated. We hypothesized that temporal phase relation induced alteration in local testicular gonadotropin-releasing hormone (GnRH)-Gonadotropin-inhibitory hormone (GnIH) and their receptor system may modulate the testicular activity and steroidogenesis through local (paracrine and autocrine) action. To validate this hypothesis, we have checked the alterations in the expression of gonadotropin-releasing hormone receptor (GnRH-R), gonadotropin-inhibitory hormone receptor (GnIH-R) messenger RNA (mRNA), growth hormone receptor (GH-R), proliferating cell nuclear antigen (PCNA), cell communication and gap junctional proteins (14-3-3 and connexin-43 [Cnx-43]), steroidogenic factor-1 (SF-1), steroidogenic acute regulatory (StAR) protein, steroidogenic enzyme (3β-hydroxysteroid dehydrogenase [3β-HSD]) in testis as well as androgen receptor (AR) in testis and epididymis of control, 8-, and 12-hr quail. Experimental findings clearly indicate the increased expression of GnIH-R mRNA and suppression of GnRH-R, GH-R, PCNA, 14-3-3, Cnx-43, SF-1, StAR, 3β-HSD in testis as well as AR in testis and epididymis in 8-hr quail, while 12-hr quail exhibited the opposite results that is significantly decreased expression of GnIH-R mRNA and increased expression of GnRH-R, GH-R, PCNA, 14-3-3, Cnx-43, SF-1, StAR, 3β-HSD in testis as well as AR in testis and epididymis. The significantly increased intratesticular testosterone has been observed in the 12-hr quail while, 8-hr quail showed opposite result. Hence, it can be concluded that 12-hr quail showed significantly increased testicular activity and steroidogenesis while opposite pattern was observed in 8-hr quail.
7 citations
TL;DR: It may be concluded that PQ treatment alters the photoperception via altering the expression of photoreceptors and also modulates the HPG axis thereby alters the reproductive functions in Japanese quails.
Abstract: Paraquat (PQ) being a potent herbicide, causes toxic effect on growth, development and reproduction of plant as well as in animals. In this study we have mainly focused on the toxic effect of PQ on photoperception via different photoreceptors present in retina, pineal and hypothalamus and thereby its effect on hypothalamic – pituitary – gonadal (HPG) axis. PQ was administered i.p.10 mg/kg body weight daily for 1 week in poultry birds Japanese quail (Coturnix coturnix japonica). Our findings clearly indicated decrease in immunoreactivity of retinal and extra retinal photoreceptors (Iodopsin, rhodopsin and transducin) following PQ treatment in comparison to control group. Increased immunoreactivity of GnIH was observed in testis and epididymis of PQ treated group. Decreased mRNA expression of photoreceptors (rhodopsin and melanopsin), steroidogenic genes, androgen receptor, GnRH-I were found in PQ treated group while increased mRNA expression of melatonin receptors (Mel 1a R, Mel 1b R, Mel 1c R) and GnIH were found in PQ treated group. Thus, from the present results it may be concluded that PQ treatment alters the photoperception via altering the expression of photoreceptors and also modulates the HPG axis thereby alters the reproductive functions in Japanese quails.
5 citations