Showing papers by "Chang Hwa Jung published in 2004"

Purification of Phosvitin from Egg Yolk and Determination of Its Physicochemical Properties

Abstract: This study was carried out to isolate phosvitin from egg yolk and to investigate its physicochemical properties as well as its functionalities. The SDS-PAGE patterns revealed that phosvitin was successfully isolated and purified from egg yolk by the NaCl sedimentation method. The phosphorus and calcium contents in phosvitin were 8.2 and 2.9%, respectively. Serine composed 35% of the total amino acids in phosvitin. When 1.0% of phosvitin was incubated with 0.1% of Ca at an ileum condition followed by centrifugation and dialysis, 44.1% of the added Ca remained soluble in the supernatant, while only 0.1% of the added Ca was found soluble in a supernatant without phosvitin addition.