Charles R. Allerson

TL;DR: This is the first report of such a potent fully modified siRNA motif, consisting entirely of 2'-O-methyl and 2'-fluoro nucleotides, which may represent a useful design for therapeutic oligonucleotides.

TL;DR: The study of the antisense strand of siRNAs demonstrated that activity depends on the position of the modifications in the sequence, and guidelines to design effective and stable si RNAs for RNA interference mediated therapeutic applications are provided.

TL;DR: In this paper, the authors provided first and second oligomer/protein compositions comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC).

TL;DR: The potency of second generation antisense oligonucleotides in animals was increased 3- to 5 -fold (ED(50) approximately 2-5 mg/kg) without producing hepatotoxicity, by employing novel nucleoside modifications that combine structural elements of 2'-O-methoxyethyl residues and locked nucleic acid.

TL;DR: The results highlight the importance of the ribo configuration 2'- and 3'-hydroxy pharmacophores for inhibition of HCV RNA replication in the cell-based assay and demonstrate that inclusion of the 2'-C-methylribonucleoside pharmacophore leads to increased resistance to adenosine deaminase and purine nucleoside phosphorylase mediated metabolism.