Christoph Busse

TL;DR: In this paper, the authors provide an overview of current and emerging sensors for bioprocess monitoring and control, including sensors directly interfaced to bioreactors for measuring important variables from the gas phase, such as oxygen and carbon dioxide concentration, and sensors for the monitoring of the biomass concentration and morphology and of the changing medium composition.

TL;DR: An overview of current and emerging sensors for single‐use bioreactors, organized by the type of interface of the sensor systems to the bioreactor, with a major focus on non‐invasive, in‐situ sensors that are based on electromagnetic, semiconducting, optical, or ultrasonic measurements.

TL;DR: Investigation of stress‐related product release from the cell surface showed no significant differences between the disposable bag reactor with wave‐induced mixing and the stirred tank reactor, and efficient and time‐saving downstream processing characterization of the final product showed a protein content below 0.04 mgprotein/gpolySia and a maximal chain length of ∼90 degree of polymerization.

TL;DR: The here presented measuring chamber, combined with an automation platform enable an accelerated integration of PAT during all stages of process development for any reactor type.

TL;DR: The ISICOM is an in situ combi-sensor that is measuring the cell-specific oxygen uptake rate (qOUR) online and contributes to a higher process understanding by offering an online view on to the cell behavior.