Showing papers by "Cornelius Faber published in 2005"
TL;DR: Results show that high-field MRI allows tracking of a minimal number of cells in vivo, well below the number used in previous studies, opening the possibility of gaining new insights into cell migration and differentiation.
123 citations
TL;DR: SOLO is the first method that achieves localization of NMR signal with a single gradient pulse and lends itself to a wide range of applications including in vivo NMR, used in conventional NMR spectrometers with one-axis gradient systems.
21 citations
Journal Article•
TL;DR: Ex vivo high-resolution MR microscopy of the spinal cord at 17.6 T allows rapid and highly accurate determination of CNS inflammation by demonstrating virtually all histologically detectable white matter inflammatory lesions.
Abstract: BACKGROUND AND PURPOSE: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disorder of the CNS and an animal model of multiple sclerosis. We used high-field MR microscopy at 17.6 T to image spinal cord inflammatory lesions in the acute stage of chronic relapsing rat EAE. We sought to compare lesions detected on MR imaging with histopathologic findings and to quantify the inflammatory lesion load. METHODS: Imaging of fixed spinal cord specimens was performed by using a 3D gradient-echo sequence with a spatial resolution of 35 × 35 × 58 μm 3 and a total imaging time of 5.5 hours. Histopathologic analysis was performed by staining axial sections with hematoxylin-eosin or Luxol fast blue to identify cellular infiltration and demyelination. RESULTS: Clinical signs of EAE occurred on days 10–14 after immunization. On day 22, healthy white matter and gray matter were differentiated by high contrast on T2*-weighted images, with white matter lesions appearing as hyperintense areas in the normal-appearing white matter. Inflammatory lesions identified on histopathologic evaluation were readily detected with MR imaging and vice versa. MR imaging and histopathologic analysis had excellent correlation regarding the extent of white matter lesions. Inflammatory infiltrates of gray matter were not detectable with MR imaging. Using a semiautomatic segmentation of the acquired MR data, we could quantify white matter lesion load. CONCLUSION: Ex vivo high-resolution MR microscopy of the spinal cord at 17.6 T allows rapid and highly accurate determination of CNS inflammation by demonstrating virtually all histologically detectable white matter inflammatory lesions.
19 citations
TL;DR: The present work aims to contribute towards the development of a coherent theory of proteinfaltung and its application in the context of sports medicine.
Abstract: Martin Horstmann, Thilo Kamphausen, Kristian Schweimer, Michael Steinert, Jorg Hacker, Axel Haase, Paul Rosch, Gunter Fischer & Cornelius Faber* Lehrstuhl fur Experimentelle Physik 5, Universitat Wurzburg, Germany; Max-Planck-Forschnungsstelle fur Enzymologie der Proteinfaltung, Halle, Germany; Lehrstuhl fur Biopolymere, Universitat Bayreuth, Germany; Institut fur Molekulare Infektionsbiologie, Universitat Wurzburg, Germany
6 citations
TL;DR: The Molekulare and zellulare MRT-Bildgebung as discussed by the authors stellt die In vivo Charakterisierung and Messung von biologischen Prozessen auf der zellulearn and molekularen Ebene dar.
Abstract: Zusammenfassung Die molekulare Bildgebung stellt “die In vivo Charakterisierung und Messung von biologischen Prozessen auf der zellularen und molekularen Ebene dar” und ermoglicht die Abbildung molekularer Abnormalitaten bei Krankheiten lange bevor erste morphologische Veranderungen auftreten. Im Augenblick beobachten wir einen raschen Zuwachs in der Anwendung der Magnet Resonanz Tomographie (MRT) fur die molekulare und zellulare Bildgebung. Die MRT erscheint als auserst attraktive Kandidatin, da die heutigen MRT Protokolle bereits anatomische, funktionelle und biochemische Informationen in ausgezeichneter Bildqualitat und in hoher raumlicher Auflosung liefern. Die Verknupfung dieser hochaufgelosten Bildgebungsmodalitat mit spezifischen MRT Kontrastmitteln fur die molekulare Bildgebung ist im Augenblick der Forschungsschwerpunkt in vielen Labors weltweit. Dieser Beitrag fasst die Grundprinzipien der molekularen MRT zusammen und beschreibt die wesentlichen Charakteristika moderner molekularer MRT Bildgebungsstrategien. Abschliesend wird ein Einblick in das wachsende Feld von moglichen Anwendungen gegeben, wie z.B. die Stammzell Bildgebung, die Apoptose Darstellung, die Plaquedetektion und die Darstellung anderer interessanter biologischer Zielstrukturen.
5 citations