D
Daisuke Kawana
Researcher at Kōchi University
Publications - 6
Citations - 240
Daisuke Kawana is an academic researcher from Kōchi University. The author has contributed to research in topics: Superoxide & Formazan. The author has an hindex of 5, co-authored 6 publications receiving 219 citations.
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Journal ArticleDOI
Spectrophotometric Assay for Superoxide Dismutase Based on the Reduction of Highly Water-soluble Tetrazolium Salts by Xanthine-Xanthine Oxidase
TL;DR: WST-1 was found more useful than WST-8 because it had shown higher sensitivity which was apparently not dependent on the assay pH value in the range pH 8.0-10.2, suggesting that these WSTs were not reduced with XO.
Journal ArticleDOI
Flow-Injection Assay of Superoxide Dismutase Based on the Reduction of Highly Water-Soluble Tetrazolium
TL;DR: In this paper, a flow-injection analysis for superoxide dismutase (SOD) activity was developed based on the use of tetrazolium salt, WST-1 (4-[3-(4-iodophenyl)-2-(4-)nitrophenyl]-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) and an enzyme reactor packed with Sepharose 4B on which xanthine oxidase (XO) and catalase were co-immobilized.
Journal ArticleDOI
Application of Novel Assay Methods for Superoxide Anion-Scavenging Activity to Food Samples.
Hiroyuki Ukeda,Hironori Moriyama,Daisuke Kawana,Yasuyuki Katayama,Kinichi Nakabayashi,Masayoshi Sawamura +5 more
TL;DR: It is confirmed that SOSA-SOSA, SOD, and ERSR have agreed on a plan to develop a new EMMARM, codenamed WST-1.
Journal ArticleDOI
Enzymatic assay of dehydrogenase substrate based on the detection of superoxide anion.
TL;DR: In this paper, a linear curve was obtained within the range of 0.5 μM −50 μM NADH concentration, which was determined by chemiluminescence using lucigenin specific for O2− instead of WST-1.