David J. Harvey

TL;DR: A method involving their conversion into methyl esters has been developed that produces strong positive-ion signals from N-linked oligosaccharides containing sialic acid and from gangliosides, which are stable, even in the presence of alpha-cyano-4-hydroxycinnamic acid.

TL;DR: The utility of ion trap mass spectrometry is indicated towards the facile determination of oligosaccharide composition, sequence, branching and linkage, providing a wealth of structural information not obtainable by other individual methods of carbohydrate mass Spectrometric analysis.

TL;DR: This paper reviews methods for the analysis of N‐linked glycans by mass spectrometry with emphasis on studies conducted at the Oxford Glycobiology Institute, and describes methods based on matrix‐assisted laser desorption/ionization (MALDI) and electrospray ionization for producing fragment ions.

TL;DR: This technique presents a complementary method to existing methodologies for the profiling of glycan mixtures by CE with on-line electrospray ion trap mass spectrometry.

TL;DR: N-Linked glycans were ionized from several matrices with a Shimadzu-Biotech AXIMA-QIT matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometer and the proposed structures of several cross-ring cleavage ions were confirmed and it was shown that MS3 spectra could be obtained from as little as 10 fmol of glycan.