D
De-Huai Zhang
Researcher at Kunming University of Science and Technology
Publications - 14
Citations - 181
De-Huai Zhang is an academic researcher from Kunming University of Science and Technology. The author has contributed to research in topics: Ganoderic acid & Lanosterol. The author has an hindex of 6, co-authored 14 publications receiving 131 citations.
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Journal ArticleDOI
Overexpression of the Squalene Epoxidase Gene Alone and in Combination with the 3-Hydroxy-3-methylglutaryl Coenzyme A Gene Increases Ganoderic Acid Production in Ganoderma lingzhi
TL;DR: The squalene epoxidase gene from the biosynthetic pathway of ganoderic acid was cloned and overexpressed in Ganoderma lingzhi, indicating that SE stimulates GA accumulation.
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Improved polysaccharide production in a submerged culture of Ganoderma lucidum by the heterologous expression of Vitreoscilla hemoglobin gene.
TL;DR: The potential of VHb to enhance G. lucidum polysaccharide production by large scale fermentation is highlighted by the observation of Vhb specific CO-difference spectrum with a maximal absorption at 419 nm for the transformant.
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Enhancement of ganoderic acid production by constitutively expressing Vitreoscilla hemoglobin gene in Ganoderma lucidum.
TL;DR: The Vitreoscilla hemoglobin gene was expressed in Ganoderma lucidum to enhance antitumor ganoderic acid (GA) production and the effects of VHb expression on the accumulation of GAs and lanosterol (intermediate) and the transcription of GA biosynthesis genes were investigated.
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Overexpression of the homologous lanosterol synthase gene in ganoderic acid biosynthesis in Ganoderma lingzhi.
TL;DR: It is demonstrated that OE of the homologous LS gene can enhance lanosterol accumulation and a large precursor supply promotes GA biosynthesis.
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Increased production of ganoderic acids by overexpression of homologous farnesyl diphosphate synthase and kinetic modeling of ganoderic acid production in Ganoderma lucidum.
TL;DR: Analysis of the kinetic process showed that FPS gene overexpression had a stronger positive impact on GA production compared with its influence on cell growth, and the developed mathematical models are useful for developing a better GA production process in future large-scale bioreactors.