The invention discloses a pair of specific primers for detecting Bergeyella, a kit and a PCR detection method. The primers comprise an upstream primer Bergeyella-F: 5'-TTGAAAGCTCCGGCGGATAG-3' and a downstream primer Bergeyella-R: 5'-ACCCTCACGAGAGTAGGTTT-3'. According to the specific primers, the kit and the PCR detection method, streptococcus, bacillus erysipelatos-suis, pasteurella multocida, salmonella, haemophilus parasuis, infectious actinobacillus pleuropneumoniae, stenotrophomonas maltophilia, pig Bergeyella and a Bergeyella zoohelcum 16S rRNA gene sequence are downloaded from a GenBankdatabase; sequence comparison is carried out through MEGA 5.05 software to search a Bergeyella 16S rRNA specific sequence; and Primer-BLAST design is utilized to amplify a pair of specific primers ofBergeyella; and a Bergeyella PCR rapid detection method is established to identify Bergeyella. The method has the advantages of being rapid, convenient, high in sensitivity and strong in specificity.

Deng Tongwei

Papers

Pair of specific primers for detecting Bergeyella, kit and PCR detection method