The invention discloses a stabilizer for free DNA and a blood sampling tube for free DNA detection. The stabilizer comprises a first solution and a second solution, so that the free DNA in blood can be stored stably at normal temperature, wherein the first solution comprises a first buffering liquid, a first anticoagulant, a cell stabilizer, a metabolic inhibitor and a first solvent; the second solution comprises a second buffering liquid, a second anticoagulant, a blood biocompatible reagent, a free DNA stabilizer and a preservative. In the mode, the free DNA in the blood can be stored stablyat the normal temperature in the implementing mode provided by the invention.

Stabilizer for free DNA and blood sampling tube for free DNA detection

The invention provides a long-duration blood cell stabilizer. The stabilizer is composed of 20-40 mumol/L disodium cromoglycate, 0.12-0.16 mg/L poloxamer, 120-140 mumol/L beta-cyclodextrin, 10-12 mumol/L ectoin, 20-25 mumol/L triflusal, 0.07% potassium dichromate and 80-200 mumol/L trehalose, wherein the potassium dichromate is in a mass percent concentration. According to the blood cell stabilizer, it is very effective to stabilize blood cells in blood and prevent platelet aggregation, the blood cells can be provided with a stabilizing effect for at least 120 hours, and compared with existing products, the stabilizing duration is prolonged by at least 48 hours.

Long-duration blood cell stabilizer

The invention relates to a blood stabilizer and application thereof. The blood stabilizer is used for stabilizing a blood sample. The blood stabilizer disclosed by the invention comprises the following components according to concentrations: 10-300micromol/L of beta-cyclodextrin, 15-350micromol/L of disodium cromoglycate, 0.01%-0.5% of potassium dichromate, 5-150micromol/L of triflusal, 0.05%-1.5% of lecithin and 2.5-5.0mumol/L of EDTA tripotassium salt, wherein the concentration of potassium dichromate and lecithin is mass percent concentration. The blood stabilizer is capable of stabilizing hemocyte, preventing karyocyte from cracking to avoid releasing DNA in cells, and avoiding increasing the concentration of DNA outside the hemocyte; and meanwhile, the blood stabilizer disclosed by the invention is capable of preventing in vitro degradation of the DNA and preventing blood coagulation.

Deng Xingfei

Papers

Blood stabilizer and application thereof