Plantes issues de protoplastes de Pétunia

Avery’s [1] classical experiments showed that it was possible to influence cell function in procaryotes by the addition of exogenous genetic information. Analysis demonstrated that bacteria can take up DNA and integrate it into their genome by genetic recombination (see A. Tomasz, this book).

DNA-mediated genetic correction of thiamineless Arabidopsis thaliana

Genetic and evolutionary consequences of symbiosis

Publisher Summary This chapter discusses several specific areas of plant tissue cultures, such as(1) rapid clonal propagation, (2) production of androgenetic (anther- or pollen-derived) haploid tissues and plants, and (3) culture and fusion of protoplasts and their use in attempts at genetic modification. Haploid callus tissues are mitotically unstable. By the use of suitable concentrations of p-fluorophenylalanine (PFP) in tissue cultures of tobacco, it is possible to maintain stable cultures of haploid cells and to select preferentially haploid cells from the mixed populations of cells of varying ploidy. Much of the work on germ plasm preservation involves the long-term storage of, for example, seeds and pollen grains. The plant tissue cultures also offer a useful method for the long-term storage of plant genetic resources, especially in those species where seeds cannot be easily obtained or stored for long periods of time, and particularly, in cultivars where vegetative propagation is essential for the maintenance of genetic stability. Two procedures developed for the long-term storage of plant tissue cultures are the storage and culture of excised shoot meristems and the freeze preservation of callus or suspension cultures.

Dieter Heß

Papers

Transformationen an höheren Organismen

Teilung isolierter Protoplasten von Petunia hybrida