D
Dmitriy A. Gorbachev
Researcher at Russian Academy of Sciences
Publications - 6
Citations - 64
Dmitriy A. Gorbachev is an academic researcher from Russian Academy of Sciences. The author has contributed to research in topics: Ligand (biochemistry) & Chromophore. The author has an hindex of 3, co-authored 6 publications receiving 35 citations. Previous affiliations of Dmitriy A. Gorbachev include Skolkovo Institute of Science and Technology.
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Journal ArticleDOI
Red-Shifted Substrates for FAST Fluorogen-Activating Protein Based on the GFP-Like Chromophores
Natalia V. Povarova,Snizhana O. Zaitseva,Nadezhda S. Baleeva,Alexander Yu. Smirnov,Ivan N. Myasnyanko,Marina B. Zagudaylova,Nina G. Bozhanova,Dmitriy A. Gorbachev,Dmitriy A. Gorbachev,Kseniya K. Malyshevskaya,Kseniya K. Malyshevskaya,Alexey S. Gavrikov,Alexander S. Mishin,Mikhail S. Baranov,Mikhail S. Baranov +14 more
TL;DR: The proposed dye instantly stains target cellular proteins fused with FAST, washes out in a minute timescale, and exhibits higher photostability of the fluorescence signal in confocal and widefield microscopy, in contrast with previously published fluorogen:FAST complexes.
Journal ArticleDOI
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.
Konstantin S. Mineev,Konstantin S. Mineev,Sergey A. Goncharuk,Sergey A. Goncharuk,Marina V. Goncharuk,Natalia V. Povarova,Anatolii I. Sokolov,Nadezhda S. Baleeva,Alexander Yu. Smirnov,Ivan N. Myasnyanko,Dmitry A. Ruchkin,Sergey Bukhdruker,Alina Remeeva,Alexey Mishin,Valentin Borshchevskiy,Valentin Borshchevskiy,Valentin Gordeliy,Alexander S. Arseniev,Dmitriy A. Gorbachev,Alexey S. Gavrikov,Alexander S. Mishin,Mikhail S. Baranov,Mikhail S. Baranov +22 more
TL;DR: The shortened FAST is designed, which is composed of only 98 amino acids, the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, by truncating 26 N-terminal residues.
Journal ArticleDOI
The Role of N-Substituents in Radiationless Deactivation of Aminated Derivatives of a Locked GFP Chromophore
Nadezhda S. Baleeva,Snezhana O. Zaitseva,Dmitriy A. Gorbachev,Alexander Yu. Smirnov,Marina B. Zagudaylova,Mikhail S. Baranov +5 more
Posted ContentDOI
NanoFAST: Structure-based design of a small fluorogen-activating protein with only 98 amino acids
Konstantin S. Mineev,Konstantin S. Mineev,Sergey A. Goncharuk,Sergey A. Goncharuk,Marina V. Goncharuk,Natalia V. Povarova,Nadezhda S. Baleeva,Alexander Yu. Smirnov,Ivan N. Myasnyanko,Dmitry A. Ruchkin,Sergey Bukhdruker,Alina Remeeva,Alexey Mishin,Valentin Borshchevskiy,Valentin Borshchevskiy,Valentin Gordeliy,Alexander S. Arseniev,Dmitriy A. Gorbachev,Alexey S. Gavrikov,Alexander S. Mishin,Mikhail S. Baranov,Mikhail S. Baranov +21 more
TL;DR: In this paper, the nanoFAST tag was designed by truncating 26 N-terminal residues of the fluorogen-activating protein FAST in the apo form and in complex with fluorogen.
Journal ArticleDOI
A Surprising Photoactivity of Blue Fluorescent Protein TagBFP Allows for Super-Resolution Microscopy
Natalia V. Klementieva,Konstantin A. Lukyanov,Dmitriy A. Gorbachev,Dmitriy M. Chudakov,Elena V. Zagaynova,Alexander S. Mishin +5 more
TL;DR: It is shown that both blinking and photo activation of TagBFP can be utilized for super-resolution imaging and photoactivation to red-emitting form should be taken into account in the design of multi-channel imaging experiments involving high-power or prolonged UV illumination.