D
Dominic Trojer
Researcher at Novartis
Publications - 3
Citations - 625
Dominic Trojer is an academic researcher from Novartis. The author has contributed to research in topics: Endosome & Gene silencing. The author has an hindex of 3, co-authored 3 publications receiving 470 citations.
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Exosomes surf on filopodia to enter cells at endocytic hot spots, traffic within endosomes, and are targeted to the ER
Wolf Heusermann,Justin Hean,Justin Hean,Dominic Trojer,Emmanuelle Steib,Stefan von Bueren,Alexandra Graff-Meyer,Christel Genoud,Katrin Martin,Nicolas Pizzato,Johannes Voshol,David V. Morrissey,Samir El Andaloussi,Samir El Andaloussi,Matthew J.A. Wood,Nicole Meisner-Kober +15 more
TL;DR: A single-vesicle dye-tracing analysis in live cells shows that exosomes enter cells as intact vesicles, primarily at filopodia-active regions, and sort into endocytic vesicle circuits that are targeted to scan the ER before being directed to lysosomes.
Journal ArticleDOI
The rough endoplasmatic reticulum is a central nucleation site of siRNA-mediated RNA silencing
Lukas Stalder,Wolf Heusermann,Lena Sokol,Dominic Trojer,Joel Wirz,Justin Hean,Anja Fritzsche,Florian Aeschimann,Vera Pfanzagl,Pascal Basselet,Jan Weiler,Martin Hintersteiner,David V. Morrissey,Nicole Meisner-Kober +13 more
TL;DR: These findings demonstrate that the outer rER membrane is a central nucleation site of siRNA‐mediated RNA silencing and identifies TRBP and PACT as key factors anchoring RISC to ER membranes in an RNA‐independent manner.
Journal ArticleDOI
Systematic characterization of extracellular vesicle sorting domains and quantification at the single molecule – single vesicle level by fluorescence correlation spectroscopy and single particle imaging
Giulia Corso,Wolf Heusermann,Wolf Heusermann,Dominic Trojer,André Görgens,Emmanuelle Steib,Emmanuelle Steib,Johannes Voshol,Alexandra Graff,Christel Genoud,Yi Lee,Justin Hean,Joel Z. Nordin,Oscar P. B. Wiklander,Samir El Andaloussi,Nicole Meisner-Kober,Nicole Meisner-Kober +16 more
TL;DR: A quantitative comparison for the most commonly used sorting proteins for bioengineering of EVs is provided and a set of biophysical techniques for straightforward quantitative and qualitative characterization of fluorescent EVs to link single vesicle analysis with single molecule quantification are introduced.