Enzymatic biodiesel: Challenges and opportunities

This review describes how the versatile Candida rugosa lipases (CRL) have extended the frontiers of biotechnology. As evidenced by the current literature, CRL claims more applications than any other biocatalyst. This review comprises a detailed discussion on the molecular biology of CRL, its versatile catalytic reactions, broad specificities and diverse immobilization strategies. It also discusses its role in the food and flavour industry, the production of ice cream and single cell protein, biocatalytic resolution of life-saving pharmaceuticals, carbohydrate esters and amino acid derivatives unobtainable by conventional chemical synthesis, potent biocide making, biosensor modulations, eco-friendly approach and bioremediation, biosurfactants in detergent making, and recently, cosmetics and perfumery. ? 1998 John Wiley & Sons, Ltd.

Candida rugosa Lipases: Molecular Biology and Versatility in Biotechnology

One stop mycology

(1999). Detection and Measurement of Microbial Lipase Activity: A Review. Critical Reviews in Food Science and Nutrition: Vol. 39, No. 2, pp. 165-187.

Detection and measurement of microbial lipase activity: a review.

Cloning, expression and characterization of a cDNA encoding a lipase from Rhizopus delemar.

Revue bibliographique sur l'activite lipolytique des microorganismes (bacteries, moisissures notamment) et la specificite des lipases vis-a-vis du substrat. Applications technologiques

Microbial lipases in biotechnology

The conditions for an optimum formation of protoplasts from mycelium of Pencillium citrinum, which produces extracellular lipase, were examined. The best results were achieved using Novozyme 234 in combination with β-glucuronidase. Chitinase or β-glucuronidase alone were not able to produce protoplasts under the given conditions. For the regeneration frequency, the type of osmotic stabilizer was important. The highest regeneration frequency (84.6% protoplasts) was achieved by using 0.6 M KCl as an osmotic stabilizer. Some regenerants were more effective in producing lipase than the parent. In particular, strain no. P-8 showed a three times higher lipolytic activity than the parent strain, but this high enzyme activity was unstable after subculture.

E. M. Zboińska

Papers

Microbial lipases in biotechnology

Penicillium citrinum protoplasts: Preparation, regeneration and lipolytic activity of regenerants