Transcriptionally active chromatin.

(1982). Chromatin Structure and Gene Activity: The Role of Nonhistone Chromosomal Protein. Critical Reviews in Biochemistry: Vol. 13, No. 1, pp. 1-86.

Chromatin Structure and Gene Activity: The Role of Nonhistone Chromosomal Protein

https://www.cell.com/cell/pdf/0092-8674(80)90357-8.pdf

Butyrate and related inhibitors of histone deacetylation block the induction of egg white genes by steroid hormones

Studies of acetylation and deacetylation in high mobility group proteins. Identification of the sites of acetylation in high mobility group proteins 14 and 17

Chemical composition of nucleosomes among domains of calf thymus chromatin differing in micrococcal nuclease accessibility and solubility properties.

Digestion of isolated Friend erythroleukemic cell nuclei with DNase I under conditions which selectively destroy the DNA of transcriptionally "active" genes releases into the supernatant fraction proteins of the non-histone "High Mobility Group" (HMGs). Two of these, HMG-14 and HMG-17(identified by solubility in trichloroacetic acid, electrophoretic mobility on SDS-polyacrylamide gels and by amino acid composition) will partially inhibit the endogenous mouse cell histone deacetylase enzymes when added to in vitro assay mixtures. Other closely related proteins do not share this inhibitory ability and thus the reaction with the enzymes appears to be specific. Since these two HMG proteins appear to be preferentially associated with the "active" fraction of chromatin, these findings have important implications for possible models of eukaryotic gene regulatory mechanisms.

E.P.M. Candido

Papers

Partial inhibition of histone deacetylase in active chromatin by HMG 14 and HMG 17