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Efrain M. Ribot

Researcher at Centers for Disease Control and Prevention

Publications -  45
Citations -  5684

Efrain M. Ribot is an academic researcher from Centers for Disease Control and Prevention. The author has contributed to research in topics: Pulsenet & Pulsed-field gel electrophoresis. The author has an hindex of 26, co-authored 45 publications receiving 5418 citations.

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Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet.

TL;DR: Standardized rapid pulsed-field gel electrophoresis (PFGE) protocols for the subtyping of Escherichia coli O157:H7, Salmonella serotypes, and Shigella species are described.
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Establishment of a Universal Size Standard Strain for Use with the PulseNet Standardized Pulsed-Field Gel Electrophoresis Protocols: Converting the National Databases to the New Size Standard

TL;DR: The Salmonella serotype Braenderup strain was chosen as the universal size standard and subjected to rigorous testing in laboratories to ensure that it met the desired criteria, including coverage of a wide range of DNA fragment sizes, even distribution of bands, and stability of the PFGE pattern.
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Rapid Pulsed-Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuni

TL;DR: Comparison of PFGE patterns obtained in six laboratories showed that subtyping results obtained using this protocol are highly reproducible, whereas the most widely used current protocols require 3 to 4 days to complete.
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PulseNet USA: a five-year update.

TL;DR: PulseNet USA is the molecular surveillance network for foodborne infections in the United States, including the methodologies used and its future possibilities, and the currently preferred subtyping method is pulsed-field gel electrophoresis (PFGE), a proven highly discriminatory molecular subtyped method.