The invention relates to an asymmetrical cyanine dye, in particular to a method for preparing an asymmetrical cyanine dye and a method for detecting bovine serum albumin by the asymmetrical cyanine dye, and belongs to the technical field of the analysis of advanced technical chemistry and biological chemistry in chemical industry. The invention provides a simple and operable method for measuring trace amount of the bovine serum albumin quantitatively in a laboratory, ordinary laboratory researchers can synthesize the appropriate dye and mark a standard curve after a solution is prepared, wherein the standard curve is used for the quantitative microanalysis of the bovine serum albumin. According to the method, when 2-methyl-3-alkyl substituted benzothiazole quaternary ammonium salt is prepared, the product is collected, heated and refluxed and is cooled and filtered, and a mother solution is heated and refluxed repeatedly and is cooled and filtered, so that the product yield is increased to more than 95 percent from 20 percent. Trace amount of bovine serum albumin is detected by using a fluorescent spectrometer, so that the detection sensitivity is high, and micromole-level dye concentration can be reached; and the method for synthesizing the dye is simple, and due to the adoption of a recrystallization method, the purification efficiency is high.

Method for preparing asymmetrical cyanine dye and method for detecting bovine serum albumin by asymmetrical cyanine dye

A method for measuring protein in sludge according to the coomassie brilliant blue method belongs to the field of influencing factors in municipal sewage treatment. After being combined with protein, coomassie brilliant blue G-250 becomes cyan, the protein-pigment combined material is highest in light absorption value under a 595nm wavelength condition, and the light absorption value is in direct proportion to protein content, the method can be used for quantitative measurement of protein. The method particularly relates to the following steps: reagent preparation, standard curve measuring, sludge pretreatment, protein extraction and measuring, and the like. The method is a simple and efficient method for measuring the protein content in sewage.

Method for measuring protein in sludge according to coomassie brilliant blue method

The invention relates to a method for detecting content of protein in a fiber A Coomassie brilliant blue approach is applied to quantitative detection on the content of protein in the fiber Coomassie brilliant blue G-250 is red under a free state; after the G-250 is combined with an alkaline amino acid and an aromatic amino acid residue of the protein in a dilute acid solution, the color becomes blue, the maximum absorption wavelength becomes 595nm from 465nm, the protein ranges from 1 microgram to 1000 micrograms, the absorbance of a protein-pigment conjugate under the wavelength of 595nm is in direct proportion to the content of protein, and the relationship conforms to the Lambert-Beer law, so that the Coomassie brilliant blue can be used for quantitatively determining the content of protein in the fiber The detection process comprises the following steps: preparing a protein standard solution, preparing a Coomassie brilliant blue solution, drawing a standard curve, obtaining the equation of linear regression and the linear correlation coefficient r, preparing a test product solution, determining the content of protein in a sample, and calculating and analyzing the result

Method for detecting content of protein in fiber

The invention discloses a method for measuring the true protein content of corn stalk fermentation feed By utilizing Coomassie brilliant blue, absorbance values under different conditions are measured and recorded, a standard curve is drawn, the protein content of a sample is calculated, and according to changes of absorbance, the digestion effect of the sample is measured Compared with the prior art, the measurement method has the advantages that an optimized precipitation method is adopted for implementation, the true protein content of the feed can be effectively measured, nonprotein interference is eliminated at the same time, and the effect is good; the measurement method is easy to operate, an expensive large instrument is not needed, needed cost is low, and the method can be widely applied to inspection in actual production of feed factories

Method for measuring true protein content of corn stalk fermentation feed

The invention discloses a method for efficiently screening saline-alkaline tolerant rice. The method comprises the following steps: A, respectively planting rice to-be-screened in multiple fields withsame texture; B, respectively dividing each field into four zones, and respectively irrigating seawater which is not diluted, 0.3 percent of diluted seawater, 0.6 percent of diluted seawater and 0.9percent of diluted seawater; C, recording growth situations, and ensuring the salinity of the irrigated seawater; D, randomly selecting plants for carrying out protein content determination during a harvesting period. According to the new method for efficiently screening the saline-alkaline tolerant rice, disclosed by the invention, influence to soluble protein contents of rice under different salinity treatment can be displayed; if the protein content of the saline-alkaline tolerant rice has no remarkable difference, the result shows that cell activity is not reduced and is consistent with ahigh yield situation, and the rice really has saline-alkaline tolerance.

Method for efficiently screening saline-alkaline tolerant rice

The invention relates to a method for analyzing protein content in 2-keto-L-gulonic acid, which comprises the following steps: adopting an optimized Coomassie brilliant blue quantitative protein method (a Bradford method), preparing phosphate buffer solution, bovine serum protein standard solution and Coomassie brilliant blue G-250 staining agent, adding the Coomassie brilliant blue G-250 stainingagent into the bovine serum protein standard solution, drawing a standard curve according to absorbency added values after the Coomassie brilliant blue is combined with the protein, then adding the 2-keto-L-gulonic acid into a Coomassie brilliant blue reagent, and calculating the protein content in the 2-keto-L-gulonic acid according to the absorbency added values and the slope coefficient of a protein concentration standard curve. The analyzing method is simple, convenient and sensitive, has the advantages of good stability, high accuracy, good repeatability and low requirement on detection equipment, is quite suitable for analyzing and detecting the protein content in the 2-keto-L-gulonic acid in the industrial production.

Fengjie Wang

Papers

Method for detecting protein content in 2-keto-L-gulonic acid