Showing papers by "Francesco Salvatore published in 1984"

The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species

Abstract: A recombinant M13 clone (O42) containing a 65 b.p. cDNA fragment from human fetal liver mRNA coding for glyceraldehyde-3-phosphate dehydrogenase has been identified and it has been used to isolate from a full-length human adult liver cDNA library a recombinant clone, pG1, which has been subcloned in M13 phage and completely sequenced with the chain terminator method. Besides the coding region of 1008 b.p., the cDNA sequence includes 60 nucleotides at the 5'-end and 204 nucleotides at the 3'-end up to the polyA tail. Hybridization of pG1 to human liver total RNA shows only one band about the size of pG1 cDNA. A much stronger hybridization signal was observed using RNA derived from human hepatocarcinoma and kidney carcinoma cell lines. Sequence homology between clone 042 and the homologous region of clone pG1 is 86%. On the other hand, homology among the translated sequences and the known human muscle protein sequence ranges between 77 and 90%; these data demonstrate the existence of more than one gene coding for G3PD. Southern blot of human DNA, digested with several restriction enzymes, also indicate that several homologous sequences are present in the human genome.

Studies on metal carbonate equilibria. Part 10. A solubility study of the complex formation in the uranium(VI)–water–carbon dioxide (g) system at 25 °C

Abstract: The complex formation in the system UVI–H2O–CO2(g) was studied by measuring the solubility of UO2(CO3)(s) as a function of the CO2 pressure and the hydrogen-ion concentration. The measurements were made at 25 °C, by using two different ionic media, 0.5 and 3 mol dm–3 NaClO4, respectively. The equilibrium constants for the following reactions were determined. Interaction UO2(CO3)(s)⇌ UO22++ CO32– Ks,o UO22++tCO32–⇌ UO2(CO3)t, t= 1–3 βt,1 3UO22++ 6CO32–⇄[(UO2)3(CO3)6]6–β6,3 coefficients Iµ(i,j) for the various uranium species with ClO4–(or Na+) were determined by using the Bronsted, Guggenheim, Scatchard specific ion-interaction theory. By using these and equilibrium data from the literature, we propose the following set of ‘best’ estimates of equilibrium constants, referred to the pure water reference state: log Ks,o=–14.4 ± 0.1, log β1,1= 9.5 ± 0.2, log β2,1= 16.6 ± 0.2, log β3,1= 21.3 ± 0.2, and log β6,3= 53.4 ± 0.8.

Isolation and nucleotide sequence of a full-length cDNA coding for aldolase B from human liver

Abstract: Two recombinant clones, pA2 and pA3, containing cDNA sequences for human aldolase B have been isolated from a full length human liver cDNA library. The larger one, pA3, has been subcloned in M13 phage and completely sequenced with the chain terminator method. The sequence covers 1,600 nucleotides including the whole coding region (1,050 nucleotides), 67 nucleotides from the 5' non-coding region and the whole 3' non-coding region, 440 nucleotides long, down to the poly-A tail. Comparison with rabbit aldolase A and with a partial sequence of rat aldolase B, shows a homology of about 76% for aldolase A and of about 94% for aldolase B, which indicates that the sequenced cDNA codes for the liver isoenzyme. This is the first complete sequence reported for human aldolase B. The pA3 clone strongly hybridizes to 18S mRNA from human adult liver as expected from the size of the isolated cDNA.

Serum pseudouridine as a biochemical marker in the development of AKR mouse lymphoma.

Abstract: Pseudouridine is a modified nucleoside derived from the degradation of some species of RNA, primarily transfer RNA, the level of which is elevated in biological fluids of tumor-bearing subjects. In order to study the relationship between pseudouridine levels and the development and progression of neoplasia, we have measured pseudouridine levels in the serum of inbred mice with high (AKR) and low (BALB/c) incidence of spontaneous lymphoma and in mice carrying transplantable lymphoid tumors. Our results show that the serum level of pseudouridine: (a) in healthy mice, is higher in females than in males; (b) increases significantly in female AKR mice in the period preceding the development of lymphoma (preneoplastic period occurring at about 6 months of age); and (c) is highest in AKR mice with lymphoma, the most elevated levels being found in mice with widely disseminated disease. The latter observation was confirmed by experiments with a transplantable AKR lymphoma (T2), where a positive correlation between tumor burden and serum pseudouridine levels was found. On the contrary, in BALB/c mice carrying a transplantable myeloma tumor (MOPC-460), no increase was seen despite the presence of a considerable tumor burden. The increase of pseudouridine in the preneoplastic period, in the absence of overt disease is viewed as an early sign of the development of the disease.