Showing papers by "Francis V. Chisari published in 1972"

Response of Monkeys to Immunization with Cholera Toxoid, Toxin, and Vaccine: Reversion of Cholera Toxoid

Abstract: Rhesus monkeys have a severe, local reaction to a 50-ag subcutaneous dose of formalinized, purified cholera toxoid The reaction was delayed, reached a maximum seven to nine days after injection, and was characterized by local edema and erythema, with prominent cellular infiltration in a later, accentuated phase of the reaction The reaction, which was similar histologically to that produced by an injection of cholera toxin, was prevented by the injection of antitoxin with the toxoid Also, no reaction to toxoid was seen either in monkeys passively immunized with antitoxin given intravenously or in actively immunized monkeys that had circulating endogenous antitoxin at the time of booster injection It was concluded that reversion of cholera toxoid to toxin in vivo caused the reaction A sustained rise in titers of antitoxin in the serum was produced by the toxoid A marked polymorphonuclear cellular response to conventional cholera vaccine with formation of local microabscess was also observed

Parathyroid necrosis and hypocalcemic tetany induced in rabbits by L-asparaginase.

Abstract: Fifty percent of New Zealand white rabbits became profoundly weak, had generalized seizures and died between 22 and 47 hours after an intravenous injection of 1000 IU/kg of L-asparaginase. The biochemical correlate of this syndrome is severe hypocalcemia associated with marked, single cell, oxyphilic necrosis in the parathyroid glands. Although survivors remained clinically well, they also developed hypocalcemia and parathyroid necrosis but to a lesser degree. Rabbits given an equivolumetric amount of saline did not develop alterations in any of these parameters. L-Asparaginase, therefore, exerts a direct toxic effect on the parathyroid glands of rabbits. The implications of this finding for man are briefly discussed.