Plants are one of the best sources to obtain a variety of natural surfactants in the field of green synthesizing material. Sambucus ebulus, which has unique natural properties, has been considered a promising material in traditional Asian medicine. In this context, zinc oxide nanoparticles (ZnO NPs) were prepared using S. ebulus leaf extract, and their physicochemical properties were investigated. X-ray diffraction (XRD) results revealed that the prepared ZnO NPs are highly crystalline, having a wurtzite crystal structure. The average crystallite size of prepared NPs was around 17 nm. Green synthesized NPs showed excellent absorption in the UV region as well as strong yellow-orange emission at room temperature. Prepared nanoparticles exhibited good antibacterial activity against various organisms and a passable photocatalytic degradation of methylene blue dye pollutants. The obtained results demonstrated that the biosynthesized ZnO NPs reveal interesting characteristics for various potential applications in the future.

https://www.mdpi.com/2076-3417/10/10/3620/pdf

Preparation and Characterization of Zinc Oxide Nanoparticles Using Leaf Extract of Sambucus ebulus

ZnO nanoparticles are still a hot area for research even after ample work has been done by researchers across the world. It is a versatile material for doping of different transition metals among o...

Progress on Transition Metal-Doped ZnO Nanoparticles and Its Application

In the present work, chemically synthesized ZnO-NPs were compared with the biosynthesized ZnO-NPs using aqueous leaf extract of Solanum nigrum (5, 10, 15 and 20 ml), at various concentrations. Different techniques like, UV–vis spectroscopy (UV–vis), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Particle size (DLS), Zeta potential (ZE), High resolution transmission electron microscope (HR-TEM) and Fourier transform infrared spectroscopy (FT-IR) were utilized to characterize the synthesized ZnO-NPs. FT-IR study confirmed the presence of falvonoids and proteins in S. nigrum leaf extract, which were the powerful reducing agents responsible for the reduction of zinc acetate salt. The antibacterial and antioxidant activity of Chem ZnO-NPs and biosynthesized ZnO-NPs were investigated with various Gram positive and Gram negative microbes utilizing disk diffusion technique. The obtained result clearly confirmed the biosynthesized ZnO-NPs potentially played as antimicrobial agent against all tested microorganism and a strong antioxidant activity against DPPH free radical scavenging. Photocatalytic degradation of synthesized ZnO-NPs were also evaluated for methylene blue dye degradation under sunlight irradiation and was confirmed that biosynthesized ZnO-NPs had high degradation compared to Chem ZnO-NPs.

Effect of chemically synthesis compared to biosynthesized ZnO-NPs using Solanum nigrum leaf extract and their photocatalytic, antibacterial and in-vitro antioxidant activity

This work evaluates the effects of different concentrations of Hibiscus sabdariffa flower (Jamaica) extracts on the green synthesis of zinc oxide (ZnO), for the photocatalytic degradation of methylene blue (MB). Zinc nitrate is used for the synthesis of ZnO as a source of the zinc ions. Extracts of 1%, 4% and 8% (% weight-volume) of Hibiscus sabdariffa, in an aqueous medium, were used as reducing and stabilizing agents. In FTIR characterization, the Zn O bond was observed at 618 cm−1. By means of XRD, the material was observed to have a hexagonal crystalline phase (Wurzite). Through XPS, the energy values of 1022 eV for Zn and 531 eV for O were observed, showing the chemical state of Zn+2. The morphology of the ZnO nanoparticles (NPs) varies in its semicircular shape and size distributions depending on the extract used, which range from 30 to 8 nm. The values of the band gap decreased from 2.96 to 2.77 eV as the concentration of extract increased. These materials presented good photocatalytic activity, degrading 97% of MB in 150 min, which are efficient results comparable to ZnO NPs green synthesized via other extracts and other methods.

Study on the effect of the concentration of Hibiscus sabdariffa extract on the green synthesis of ZnO nanoparticles

The triad of problem leading on the diabetic foot is neuropathy, vascular changes and infections, which constitute the diabetic foot syndrome 3 . Infection complicates the pathological picture of diabetic foot and plays a main role in development of moist gangrene. This study was done to detect the prevalence of diabetic foot, to know the risk factors for developing diabetic foot ulcers, microbial profile in diabetic wound, common susceptibility pattern to antibiotics and presence of multiple drug resistant organisms in diabetic foot.

/pdf/a-clinico-microbiological-study-of-diabetic-foot-ulcers-2kxg4fnfpv.pdf

A Clinico Microbiological Study of Diabetic Foot Ulcers

Enhanced antibacterial effects of green synthesized ZnO NPs using Aristolochia indica against Multi-drug resistant bacterial pathogens from Diabetic Foot Ulcer

Synthesis and characterization of ZnO phytonanocomposite using Strychnos nux-vomica L. (Loganiaceae) and antimicrobial activity against multidrug-resistant bacterial strains from diabetic foot ulcer

Potential bactericidal activity of S. nux-vomica-ZnO nanocomposite against multidrug-resistant bacterial pathogens and wound-healing properties.

Chemical composition and antibacterial activity of pajanelia longifolia (willd.) k.schum against multi drug resistant clinical isolates from diabetic foot ulcer.

Objectives AmpC β-lactamases are cephalosporinases that confer resistance to cephalothin, cefazolin, cefoxitin, penicillin, and β-lactamase inhibitor-β-lactam combinations. Even though the AmpC resistance is reported, but the accurate occurrence of AmpC β-lactamases in Enterobacteriaceae members is still unknown. Techniques to identify AmpC producers are still evolving but not yet optimized for the clinical laboratory. Here we aimed to compare the test performance of two different phenotypic methods, that is inhibitor-based assay using boronic acid and disk approximation test for AmpC detection in Enterobacteriaceae isolates from a tertiary hospital microbiology laboratory. Materials and Methods The study includes 137 nonrepeat Enterobacteriaceae strains. Bacterial isolates, that yielded a zone diameter of less than 18 mm for cefoxitin by disk diffusion method were considered potential AmpC producers and further confirmed by phenotype methods—inhibitor-based assay using boronic acid and disk approximation test. A multiplex polymerase chain reaction was used to detect the most common plasmid-mediated AmpC genes: ACC, FOX, MOX, DHA, CIT, and EBC. Results Of the 137 clinical isolates, 58 (42.33%) were cefoxitin resistant, while 53.4 and 18.9% of the cefoxitin-resistant isolates were positive by inhibitor-based assay and disk approximation test. Multiplex PCR detected 42 (30.6%) isolates with AmpC genes. Of the 42 isolates, the inhibitor-based assay detected 25 (59.5%) isolates, while the disk approximation test detected nine (21.4%) isolates. Conclusion Our findings suggest that inhibitor-based assay using boronic acid can be used for the detection of the isolates that harbor AmpC β-lactamases. This method is cost-effective, simple to perform, and easy to interpret. Thus AmpC detection as a routine in clinical laboratories can help in appropriate therapeutic intervention and improved infection control.

/pdf/evaluation-of-two-phenotypic-methods-for-the-detection-of-3my8rj6kyw.pdf

Ganesan Shanthi

Papers

Enhanced antibacterial effects of green synthesized ZnO NPs using Aristolochia indica against Multi-drug resistant bacterial pathogens from Diabetic Foot Ulcer

Synthesis and characterization of ZnO phytonanocomposite using Strychnos nux-vomica L. (Loganiaceae) and antimicrobial activity against multidrug-resistant bacterial strains from diabetic foot ulcer

Potential bactericidal activity of S. nux-vomica-ZnO nanocomposite against multidrug-resistant bacterial pathogens and wound-healing properties.

Chemical composition and antibacterial activity of pajanelia longifolia (willd.) k.schum against multi drug resistant clinical isolates from diabetic foot ulcer.

Evaluation of Two Phenotypic Methods for the Detection of Plasmid-Mediated AmpC β-Lactamases among Enterobacteriaceae Isolates.