The invention provides an antibody detection kit of human T-lymphotropic virus (HTLV). The kit comprises magnetic micro particles which are marked by antigens of HTLV-I type virus and HTLV-II type virus, and acridinium ester, negative control, first positive control and second positive control which are coupled with the HTLV-I type virus and the HTLV-II type virus. The invention further provides an application and a preparing method of the antibody detection kit.

Antibody detection kit of human T-lymphotropic virus (HTLV) and application and preparing method thereof

The invention provides a kit for detecting the content of NMP22 (Nuclear Matrix Protein 22) and a preparation method of the kit. A chemiluminescence method of a tubular acridinium ester marker is utilized, and the content of the NMP22 in human urine is determined by adopting a full-automatic chemiluminescence immunity analyzer Caris200, so as to help meet medical requirements on early diagnosis of bladder cancer and prognosis monitoring and the like. The kit provided by the invention has remarkable advantages of high sensitivity, high specificity, good stability, simplicity and convenience for operation, low cost and the like.

Kit for detecting NMP22 (Nuclear Matrix Protein 22) and preparation method thereof

The invention provides a kit for quantitatively detecting C reactive protein and a preparation method of the kit. The kit comprises a monoclonal antibody, a carrier, a C reactive protein calibration product, a cleaning solution and a chemluminescent substrate solution, wherein the monoclonal antibody marks C reactive protein of a tracer; and the carrier is coated with the monoclonal antibody withthe C reactive protein. Meanwhile, the preparation method of the kit comprises the following steps: marking the C reactive protein of the tracer by the monoclonal antibody; coating the carrier with the monoclonal antibody with the C reactive protein; preparing a CRP calibration product by using a C reactive protein pure product; packing the C reactive protein calibration product, the monoclonal antibody for marking the C reactive protein of the tracer and a chemluminescent substrate reacted by the tracer; and assembling into a finished product. The kit is high in sensitivity, good in specificity, high in quantitative detection result accuracy, low in using cost and easy to popularize and use.

Kit for quantitatively detecting C reactive protein and preparation method

The invention relates to a chemiluminescent analysis method for detecting the concentration of hydrogen peroxide in a solution. In the analysis method, acridine sulfonamide with the same category of structure is used as a luminescence agent to measure the content of hydrogen peroxide in the solution; compared with an acridinium ester compound used as the luminescence agent, the measurement resultis more accurate and is not influenced by pH of the hydrogen peroxide solution. The chemiluminescent analysis method for detecting the concentration of hydrogen peroxide in the solution provided by the invention can be used for the detection of hydrogen peroxide content in the fields such as chemical reagents, milk, industrial water and food processing; compared with an existing hydrogen peroxidedetection technology, only a chemiluminescent detection needs to be provided, the detection cost is low, the operation is simple and convenient, the detection is rapid and efficient; and after a standard curve is established, the detection at each time can be completed in a few minutes.

Chemiluminescent analysis method for detecting concentration of hydrogen peroxide in solution

The invention relates to a screening method for a chemiluminescent reagent stabilizer. The method adopts acridine sulfonamide with a structure of one class, the consumption capacity of different stabilizers for hydrogen peroxide is investigated by a quantitative determination method of the hydrogen peroxide, wherein the stabilizers include substances with reductibility such as BSA, a reductive amino acid including glycine, lysine, histidine, and the like, a citric acid, and a TRIS buffer solution. The method enables the working intensity and time of screening the reagent stabilizer to be greatly saved; detection is fast and efficient, and each test takes only a few minutes to complete; and the method provides a reference basis for selection and dosage of the chemiluminescence reagent stabilizer.

Screening method for chemiluminescent reagent stabilizer

The invention discloses an enhanced acridinium ester luminescence system, which comprises a pre-activating liquid, an activating liquid, and acridinium ester linked to a to-be-detected object. After linked to the to-be-detected object, the acridinium ester linked to the to-be-detected object gives out light through actions of the pre-activating liquid, and the activating liquid in sequence. The pre-activating liquid is a solution of H2O2 containing 0.125-1% of a stabilizer and 0.125-1% of an enhancer, and the activating liquid is a solution of NaOH containing 0.125-1% of the stabilizer and 0.125-1% of the enhancer, wherein the stabilizer is dimethylformamide or ethyl alcohol, and the enhancer is Triton X-100 or Tween-20. According to the invention, the luminescent intensity of the acridinium ester is effectively enhanced, and the probability of occurrence of bubbles in the luminescence system is reduced. The freezing point of the luminescence system is lowered, so that the luminescence system is unlikely to freeze during long-time transportation in winter.

Enhanced acridinium ester luminescence system

The invention discloses a whole-course C-reactive protein assay kit and an application thereof. The kit includes the following components: an M reagent, an R1 reagent, an R2 reagent, an alkaline pre-treatment solution, a pre-excitation agent and an excitation agent. The M reagent includes 0.5-1 mg/ml of magnetic particles coated by a first antibody, 0.5-1% of casein and 0.5-1% of bovine serum albumin, wherein the solvent is a phosphate buffer solution being 7.0-8.0 in pH. The coating quantity of the first antibody is 5-15 [mu]g per mg of the magnetic particles. The R1 reagent is an HCl solution being 10-20 mM in concentration. The R2 reagent includes acridinium ester which is coated by a second antibody, 0.5-1% of casein and 0.5-1% of bovine serum albumin, wherein the solvent is a phosphate buffer solution being 7.0-8.0 in pH. The coating quantity of the second antibody is 5-15 [mu]g per ml of the acridinium ester. The alkaline pre-treatment solution is a NaOH solution being 20-50 mM in concentration. Strong alkali is added to the kit during reaction to damage the proteins, and then strong acid is added to neutralize the reaction system. The kit is 0.02-100 mg/l in detection range, and the reagent can reach the requirement of detection of the whole course of C-reactive protein.

Ge Shengxiang

Papers

Enhanced acridinium ester luminescence system

Whole-course C-reactive protein assay kit and application thereof