G
Gerald F. Joyce
Researcher at Salk Institute for Biological Studies
Publications - 172
Citations - 19903
Gerald F. Joyce is an academic researcher from Salk Institute for Biological Studies. The author has contributed to research in topics: RNA & Ribozyme. The author has an hindex of 61, co-authored 170 publications receiving 18663 citations. Previous affiliations of Gerald F. Joyce include University of California, Berkeley & University of California, San Diego.
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Journal ArticleDOI
A General Purpose RNA-Cleaving DNA Enzyme
TL;DR: An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions, and its activity is dependent on the presence of Mg2+ ion.
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Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA
TL;DR: The selected molecule represents the discovery of the first RNA enzyme known to cleave single-stranded DNA specifically and allows evolution experiments to be carried out in response to artificially imposed selection constraints.
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A DNA enzyme that cleaves RNA
TL;DR: Using in vitro selection techniques, a DNA enzyme is obtained that catalyzes the Pb(2+)-dependent cleavage of an RNA phosphoester in a reaction that proceeds with rapid turnover, and compares favorably to that of known RNA enzymes.
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Randomization of genes by PCR mutagenesis.
R C Cadwell,Gerald F. Joyce +1 more
TL;DR: A modified polymerase chain reaction (PCR) was developed to introduce random point mutations into cloned genes and was used to mutagenize the gene that encodes the Tetrahymena ribozyme with a mutation rate of 0.66% +/- 0.13% per position per PCR.
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A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron
TL;DR: The design and synthesis of a 1,669-nucleotide, single-stranded DNA molecule that is readily amplified by polymerases and that, in the presence of five 40-mer synthetic oligodeoxynucleotides, folds into an octahedron structure by a simple denaturation–renaturation procedure is reported.