Gerson Kegeles

TL;DR: Subunit interactions in the hemocyanin of New England lobster, Homarus americanus, were investigated by means of the ultracentrifuge, using sedimentation velocity and Archibald molecular weight methods to verify that a 17S species dimerizes rapidly and reversibly to form a 25S species in the pH range 9.4–9.7 in the presence of calcium ion.

TL;DR: In this paper, the shell model is extended to predict the moving boundary sedimentation behavior of micellar β-casein, and the observed sedimentation patterns, as well as the critical concentration predictions of the monomer-single polymer Gilbert sedimentation model are satisfactorily described with the present model, based on a continuous distribution of intermediates between monomers and the largest possible spherical micelles.

TL;DR: This chapter develops the subject of kinetically controlled association–dissociation reactions in a format that first considers theoretical predictions as to the shape of the sedimentation pattern and then turns to the combined application of velocity sedimentation, thermodynamic measurements, and relaxation kinetics to the characterization of the association–Dissociation of hemocyanins from different species.