Molecular imprinting technology (MIT) concerns formation of selective sites in a polymer matrix with the memory of a template. Recently, molecularly imprinted polymers (MIPs) have aroused extensive attention and been widely applied in many fields, such as solid-phase extraction, chemical sensors and artificial antibodies owing to their desired selectivity, physical robustness, thermal stability, as well as low cost and easy preparation. With the rapid development of MIT as a research hotspot, it faces a number of challenges, involving biological macromolecule imprinting, heterogeneous binding sites, template leakage, incompatibility with aqueous media, low binding capacity and slow mass transfer, which restricts its applications in various aspects. This critical review briefly reviews the current status of MIT, particular emphasis on significant progresses of novel imprinting methods, some challenges and effective strategies for MIT, and highlighted applications of MIPs. Finally, some significant attempts in further developing MIT are also proposed (236 references).

http://ir.yic.ac.cn/bitstream/133337/4853/1/Recent%20advances%20in%20molecular%20imprinting%20technology%20current%20status%2c%20challenges%20and%20highlighted%20applications.pdf

Recent advances in molecular imprinting technology: current status, challenges and highlighted applications

The detection of specific proteins as biomarkers of disease, health status, environmental monitoring, food quality, control of fermenters and civil defence purposes means that biosensors for these targets will become increasingly more important. Among the technologies used for building specific recognition properties, molecularly imprinted polymers (MIPs) are attracting much attention. In this critical review we describe many methods used for imprinting recognition for protein targets in polymers and their incorporation with a number of transducer platforms with the aim of identifying the most promising approaches for the preparation of MIP-based protein sensors (277 references).

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The rational development of molecularly imprinted polymer-based sensors for protein detection.

https://rua.ua.es/dspace/bitstream/10045/40170/3/2013_Barbosa_etal_Biomac.pdf

Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties.

Herein, we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004-2011. In total, 3779 references to the original papers, rev ...

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011.

ConspectusNature has mastered the art of molecular recognition. For example, using synergistic non-covalent interactions, proteins can distinguish between molecules and bind a partner with incredible affinity and specificity. Scientists have developed, and continue to develop, techniques to investigate and better understand molecular recognition. As a consequence, analyte-responsive hydrogels that mimic these recognitive processes have emerged as a class of intelligent materials. These materials are unique not only in the type of analyte to which they respond but also in how molecular recognition is achieved and how the hydrogel responds to the analyte. Traditional intelligent hydrogels can respond to environmental cues such as pH, temperature, and ionic strength. The functional monomers used to make these hydrogels can be varied to achieve responsive behavior. For analyte-responsive hydrogels, molecular recognition can also be achieved by incorporating biomolecules with inherent molecular recognition pro...

/pdf/analyte-responsive-hydrogels-intelligent-materials-for-2pqwg63uv1.pdf

Analyte-Responsive Hydrogels: Intelligent Materials for Biosensing and Drug Delivery

Protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels.

The objective of this study is to prepare bilirubin-imprinted polymeric particles for the selective removal of bilirubin from hyperbilirubinemic human plasma. N-methacryloyl-(l)-tyrosine methyleste...

Selective Removal of Bilirubin from Human Plasma with Bilirubin-Imprinted Particles

Bilirubin recognition via molecularly imprinted supermacroporous cryogels.

Molecular imprinted polymers are artificial, template-made materials with the ability to recognize and to specifically bind the target molecule. The aim of this study is to prepare supermacroporous cryogel with embedded bilirubin-imprinted particles which can be used for the selective removal of bilirubin from human plasma. N-methacryloyl-(L)-tyrosinemethylester (MAT) was chosen as the pre-organization monomer. In the first step, bilirubin was complexed with MAT and the bilirubin-imprinted poly(hydroxyethyl methacrylate-N-methacryloly-(L)-tyrosine methyl-ester) [MIP] monolith was produced by bulk polymerization. MIP monolith was smashed and the particles ground and sieved through 100 pm sieves. In the second step. the supermacroporous poly(hydroxyethyl methacrylate) (PHEMA) cryogel with embedded MIP particles [PHEMA/MIP composite cryogel] was produced by free radical polymerization initiated by N.N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. After that, the template (i.e., bilirubin) molecules were removed using sodium carbonate and sodium hydroxide. Compared with the PHEMA cryogel (0.2 mg/g polymer), the bilirubin adsorption capacity of the PHEMA/MIP composite cryogel (10.3 mg/g polymer) was improved significantly due to the embedded MIP particles into the polymeric matrix. The relative selectivity coefficients of PHEMA/MIP composite cryogel for bilirubin/cholesterol and bilirubin/testosterone were 8.6 and 4.1 times greater than the PHEMA cryogel, respectively. The PHEMA/MIP composite cryogel could be used many times without decreasing the bilirubin adsorption capacity significantly. (c) 2008 Elsevier Ltd. All rights reserved.

Supermacroporous poly (hydroxyethyl methacrylate) based cryogel with embedded bilirubin imprinted particles

A new generation of molecularly imprinted macroporous hydrogels, so-called cryogels, were prepared for depletion of hemoglobin from human blood. Hb-PHEMAH cryogels were prepared with gel fraction yields up to 90%, and its morphology and porosity were characterized by FTIR, SEM, swelling studies, flow dynamics and surface area measurements. Selective binding experiments were performed in the presence of competitive PHEMAH cryogel exhibited a high binding capacity and selectivity for Hb in the presence of Myb and BSA. The selectivity of Hb-PHEMAH cryogel for Hb was confirmed by HPLC. Hb depletion from blood hemolysate was also studied using SDS-PAGE. Hb-PHE-MAH cryogel can be reused many times with no apparent decrease in Hb adsorption capacity. (Less)

Gözde Baydemir

Papers

Protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels.

Selective Removal of Bilirubin from Human Plasma with Bilirubin-Imprinted Particles

Bilirubin recognition via molecularly imprinted supermacroporous cryogels.

Supermacroporous poly (hydroxyethyl methacrylate) based cryogel with embedded bilirubin imprinted particles

Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood