Catching a resurgence: Increase in SARS-CoV-2 viral RNA identified in wastewater 48 h before COVID-19 clinical tests and 96 h before hospitalizations.

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First report of natural infection of Dracaena braunii by pepper mild mottle virus in Korea

We investigated the roles of different concentrations of chemical synthetic spherical silver nanoparticles (AgNPs) in protecting pepper seedlings of the Mecca region, which were naturally and artificially infected by the pepper mild mottle virus (PMMoV). The virus shows many infection symptoms, including pepper leaf deformation with filiform leaves and severe mosaic symptoms. Our study focused on the antiviral activity of different concentrations of spherical nanoparticles in controlling PMMoV infecting pepper seedlings. PMMoV identification was confirmed via DAS-ELISA using the following antiserum: PMMoV, cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), tomato mosaic virus (ToMV), potato virus Y (PVY), and tomato spotted wilt virus (TSWV). The presence of PMMoV was confirmed using electron microscopy and reverse transcription polymerase chain reaction (RT-PCR). We evaluated the effects of exogenously applied different concentrations of AgNPs on CMV infection rate, infection severity, virus concentration, and the concentrations of photosynthetic pigments chlorophyll a, chlorophyll b, carotenoid content, phenolic compounds, and protein components in virus-infected plant cells that were treated with three different concentration of nanoparticles (200, 300, and 400 µg/L) compared to the positive and negative control.

https://www.mdpi.com/1420-3049/28/1/139/pdf?version=1671870113

Controlling Pepper Mild Mottle Virus (PMMoV) Infection in Pepper Seedlings by Use of Chemically Synthetic Silver Nanoparticles

This is a report of the natural occurrence of Capsicum frutescens (cayenne pepper) and Capsicum annuum L. (sweet pepper) infection with pepper mild mottle virus (PMMoV) in Indonesia, which was detected serologically via dot-immunobinding assay and confirmed by RT-PCR of the coat-protein–encoding gene and DNA sequencing. Phylogenetically, the cayenne and sweet-pepper isolates were closely related to Capsicum spp. isolates from Turkey, Italy and Korea, with 99.1%–99.5% and 100% identity at the nucleotide and amino acid levels, respectively. 

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Pepper mild mottle virus infection in cayenne and sweet pepper in Indonesia

/pdf/first-report-of-natural-infection-of-hydrangea-macrophylla-14hot2bt.pdf

First report of natural infection of Hydrangea macrophylla by pepper mild mottle virus in Korea

/pdf/first-report-of-natural-infection-of-tomato-by-pepper-mild-2wj5s6bzfq.pdf

Guan Lin Tan

Papers

First report of natural infection of tomato by pepper mild mottle virus in China