3 – Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures

An assembly map of 30S ribosomal subunits has been constructed. The assembly reaction is sequential and cooperative. The map may reflect the topological relationships among ribosomal proteins in the ribosomal structure.

Assembly Mapping of 30S Ribosomal Proteins from E. coli

Two-dimensional gel electrophoresis separates all of the component proteins of the ribosomal subunits of Escherichia coli. This method shows 21 proteins in the 30S, and 34 proteins in the 50S, subunit.

https://www.pnas.org/content/pnas/67/3/1276.full.pdf

Ribosomal Proteins, XII. Number of Proteins in Small and Large Ribosomal Subunits of Escherichia coli as Determined by Two-Dimensional Gel Electrophoresis

https://www.jbc.org/content/248/16/5720.full.pdf

H. Delius

Papers

Ribosomal Proteins of E. coli: Stoichiometry and Implications for Ribosome Structure