Osteoblast adhesion on biomaterials.

http://www.heavythinking.org/cp-blog/wp-content/uploads/2011/03/Flemming-et-al-1999-Effects-of-synthetic-micro-and-nano-structured-surfaces-on-cell-behaviour.pdf

Effects of synthetic micro- and nano-structured surfaces on cell behavior

http://www.ibmc.up.pt/projectos/FJMonteiro/Bonamidi%20Project/References%20Bonamidi/Curtis%20Wolkinson%20-%20Topographical%20control%20of%20cell.pdf

Topographical control of cells

Aligned biodegradable nanofibrous structure : a potential scaffold for blood vessel engineering

The human corneal basement membrane has a rich felt-like surface topography with feature dimensions between 20 nm and 200 nm. On the basis of these findings, we designed lithographically defined substrates to investigate whether nanotopography is a relevant stimulus for human corneal epithelial cells. We found that cells elongated and aligned along patterns of grooves and ridges with feature dimensions as small as 70 nm, whereas on smooth substrates, cells were mostly round. The percentage of aligned cells was constant on substrate tomographies with lateral dimensions ranging from the nano- to the micronscale, and increased with groove depth. The presence of serum in the culture medium resulted in a larger percentage of cells aligning along the topographic patterns than when no serum was added to the basal medium. When present, actin microfilaments and focal adhesions were aligned along the substrate topographies. The width of the focal adhesions was determined by the width of the ridges in the underlying substrate. This work documents that biologic length-scale topographic features that model features encountered in the native basement membrane can profoundly affect epithelial cell behavior.

/pdf/epithelial-contact-guidance-on-well-defined-micro-and-2a54rblps5.pdf

Epithelial contact guidance on well-defined micro- and nanostructured substrates

To evaluate the effect of surface treatment and surface microtexture on cellular behavior, smooth and microtextured silicone substrata were produced. The microtextured substrata possessed parallel surface grooves with a width and spacing of 2.0 (SilD02), 5.0 (SilD05), and 10 microns (SilD10). The groove depth was approximately 0.5 microns. Subsequently, these substrata were either left untreated (NT) or treated by ultraviolet irradiation (UV), radiofrequency glow discharge treatment (RFGD), or both (UVRFGD). After characterization of the substrata, rat dermal fibroblasts (RDF) were cultured on the UV, RFGD, and UVRFGD treated surfaces for 1, 3, 5, and 7 days. Comparison between the NT and UV substrata revealed that UV treatment did not influence the contact angles and surface energies of surfaces with a similar surface topography. However, the contact angles of the RFGD and UVRFGD substrata were significantly smaller than those of the UV and NT substrata. The dimension of the surface microevents did not influence the wettability characteristics. Cell culture experiments revealed that RDF cell growth on UV-treated surfaces was lower than on the RFGD and UVRFGD substrata. SEM examination demonstrated that the parallel surface grooves on the SilD02 and SilD05 substrata were able to induce stronger cell orientation and alignment than the events on SilD10 surfaces. By combining all of our findings, the most important conclusion was that physicochemical parameters such as wettability and surface free energy influence cell growth but play no measurable role in the shape and orientation of cells on microtextured surfaces.

/pdf/effect-of-parallel-surface-microgrooves-and-surface-energy-45fefok04i.pdf

Effect of parallel surface microgrooves and surface energy on cell growth.

https://repository.ubn.ru.nl/bitstream/handle/2066/22837/22837___.PDF

H. T. J. Smits

Papers

Effect of parallel surface microgrooves and surface energy on cell growth.

Quantitative analysis of cell proliferation and orientation on substrata with uniform parallel surface micro grooves