Analysis and modeling of myopia-related factors based on questionnaire survey

iEnhancer-MRBF: Identifying enhancers and their strength with a multiple Laplacian-regularized radial basis function network.

Simple Summary Breast cancer is the most fatal female cancer, which the existing clinical and pathological information sometimes fails to diagnose accurately. Recent artificial intelligence-based studies have shown the capability of identifying molecular biomarkers using high-throughput genomics data. Our aim was to apply machine learning methods to a large cohort of transcriptomics data for gene reduction and the construction of a diagnostic model for cancer classification. Advanced statistical methods and cross-validation with another set of machine learning methods increased the accuracy of the diagnostic model and predicted a novel diagnostic nine-gene signature. Further, survival analysis revealed a novel prognostic model of eight-gene signatures. Experimental validation confirmed the expression of the identified gene signatures in breast cancer patients and increased the reliability of the study. The identified gene signature biomarkers have the potential to improve healthcare management with precise diagnosis and prognosis at a reduced cost. Abstract Background: Breast cancer (BC) is one of the most common female cancers. Clinical and histopathological information is collectively used for diagnosis, but is often not precise. We applied machine learning (ML) methods to identify the valuable gene signature model based on differentially expressed genes (DEGs) for BC diagnosis and prognosis. Methods: A cohort of 701 samples from 11 GEO BC microarray datasets was used for the identification of significant DEGs. Seven ML methods, including RFECV-LR, RFECV-SVM, LR-L1, SVC-L1, RF, and Extra-Trees were applied for gene reduction and the construction of a diagnostic model for cancer classification. Kaplan–Meier survival analysis was performed for prognostic signature construction. The potential biomarkers were confirmed via qRT-PCR and validated by another set of ML methods including GBDT, XGBoost, AdaBoost, KNN, and MLP. Results: We identified 355 DEGs and predicted BC-associated pathways, including kinetochore metaphase signaling, PTEN, senescence, and phagosome-formation pathways. A hub of 28 DEGs and a novel diagnostic nine-gene signature (COL10A, S100P, ADAMTS5, WISP1, COMP, CXCL10, LYVE1, COL11A1, and INHBA) were identified using stringent filter conditions. Similarly, a novel prognostic model consisting of eight-gene signatures (CCNE2, NUSAP1, TPX2, S100P, ITM2A, LIFR, TNXA, and ZBTB16) was also identified using disease-free survival and overall survival analysis. Gene signatures were validated by another set of ML methods. Finally, qRT-PCR results confirmed the expression of the identified gene signatures in BC. Conclusion: The ML approach helped construct novel diagnostic and prognostic models based on the expression profiling of BC. The identified nine-gene signature and eight-gene signatures showed excellent potential in BC diagnosis and prognosis, respectively.

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Identification of Novel Diagnostic and Prognostic Gene Signature Biomarkers for Breast Cancer Using Artificial Intelligence and Machine Learning Assisted Transcriptomics Analysis

Accurate diagnosis is the key to providing prompt and explicit treatment and disease management. The recognized biological method for the molecular diagnosis of infectious pathogens is polymerase chain reaction (PCR). Recently, deep learning approaches are playing a vital role in accurately identifying disease-related genes for diagnosis, prognosis, and treatment. The models reduce the time and cost used by wet-lab experimental procedures. Consequently, sophisticated computational approaches have been developed to facilitate the detection of cancer, a leading cause of death globally, and other complex diseases. In this review, we systematically evaluate the recent trends in multi-omics data analysis based on deep learning techniques and their application in disease prediction. We highlight the current challenges in the field and discuss how advances in deep learning methods and their optimization for application is vital in overcoming them. Ultimately, this review promotes the development of novel deep-learning methodologies for data integration, which is essential for disease detection and treatment.

A review of multi-omics data integration through deep learning approaches for disease diagnosis, prognosis, and treatment

https://pharmacophorejournal.com/storage/files/article/8237b72c-1884-497a-826d-9def44e7db43-WpJb3CXdsvXujleX/pharma-2023-vol14-iss3-may-jun-23-34-7202.pdf

Anticancer Effect of Actinomycetes Secondary Metabolite Against Breast Cancer Cell Line (MCF-7); Cytological and Molecular Studies

Background: Breast cancer is the second largest cancer in the world, the incidence of breast cancer continues to rise worldwide, and women’s health is seriously threatened. Therefore, it is very important to explore the characteristic changes of breast cancer from the gene level, including the screening of differentially expressed genes and the identification of diagnostic markers. Methods: The gene expression profiles of breast cancer were obtained from the TCGA database. The edgeR R software package was used to screen the differentially expressed genes between breast cancer patients and normal samples. The function and pathway enrichment analysis of these genes revealed significant enrichment of functions and pathways. Next, download these pathways from KEGG website, extract the gene interaction relations, construct the KEGG pathway gene interaction network. The potential diagnostic markers of breast cancer were obtained by combining the differentially expressed genes with the key genes in the network. Finally, these markers were used to construct the diagnostic prediction model of breast cancer, and the predictive ability of the model and the diagnostic ability of the markers were verified by internal and external data. Results: 1060 differentially expressed genes were identified between breast cancer patients and normal controls. Enrichment analysis revealed 28 significantly enriched pathways (p < 0.05). They were downloaded from KEGG website, and the gene interaction relations were extracted to construct the gene interaction network of KEGG pathway, which contained 1277 nodes and 7345 edges. The key nodes with a degree greater than 30 were extracted from the network, containing 154 genes. These 154 key genes shared 23 genes with differentially expressed genes, which serve as potential diagnostic markers for breast cancer. The 23 genes were used as features to construct the SVM classification model, and the model had good predictive ability in both the training dataset and the validation dataset (AUC = 0.960 and 0.907, respectively). Conclusion: This study showed that the difference of gene expression level is important for the diagnosis of breast cancer, and identified 23 breast cancer diagnostic markers, which provides valuable information for clinical diagnosis and basic treatment experiments.

https://www.frontiersin.org/articles/10.3389/fcell.2021.811585/pdf

Haoran Jiang

Papers

Identification of Diagnostic Markers for Breast Cancer Based on Differential Gene Expression and Pathway Network