Platelets in the blood of volunteers who have taken aspirin can no longer produce prostaglandins.

Aspirin selectively inhibits prostaglandin production in human platelets

Summary: Methods have been developed for the preparation of suspensions of washed platelets from humans Heparin is used in the washing fluids to prevent: thrombin generation and apyrase is used to prevent adenine nucleotide accumulation Platelets suspended in Eagle's tissue culture medium containing albumin were more responsive to ADP than platelets in Tyrode's-albumin solution Addition of fibrinogen is required for maximum sensitivity to ADP-induced aggregation These platelets can be stored for 4 hr or more at 37°C in the presence of apyrase and maintain their ability to aggregate upon the addition of low concentrations of ADP

Without apyrase the platelets gradually become insensitive to ADP upon storage at 37°C; this is presumably caused by the accumulation of ADP in the suspending fluid because sensitivity can be partially restored by the addition of apyrase and further incubation

Preparation of Suspensions of Washed Platelets from Humans

This review is aimed at providing readers with a comprehensive reference article about the distribution and function of P2 receptors in all the organs, tissues, and cells in the body. Each section provides an account of the early history of purinergic signaling in the organ/cell up to 1994, then summarizes subsequent evidence for the presence of P2X and P2Y receptor subtype mRNA and proteins as well as functional data, all fully referenced. A section is included describing the plasticity of expression of P2 receptors during development and aging as well as in various pathophysiological conditions. Finally, there is some discussion of possible future developments in the purinergic signaling field.

/pdf/cellular-distribution-and-functions-of-p2-receptor-subtypes-32555c8g2p.pdf

Cellular distribution and functions of P2 receptor subtypes in different systems.

Polyunsaturated Fatty Acid Families and Interrelationships Omega-6 Fatty Acids and Plasma Lipids Plasma Cholesterol Plasma Triglycerlde Plasma LJpoprotelns and Apolipoproteins Hypolipldemic Mechanisms of Linoleic Acid Decrease in Cholesterol Absorption Increase in Fecal Steroid Excretion Reduced Cholesterol Synthesis and the Transfer of Cholesterol to the Tissues Decrease in Cholesterol Content of LJpoproteins Changes in the Rates of Synthesis and Catabolism of LJpoproteins Membrane Fatty Acid Composition and Upoprotein Metabolism

Haslam Rj

Papers

Role of adenosine diphosphate in the aggregation of human blood-platelets by thrombin and by fatty acids.