Hengqian Lu

TL;DR: The present findings offer a panoramic view of resource allocation by M. alpina in response to nutrient stress and revealed a set of intriguing associations between resource reallocation and TAG accumulation.

TL;DR: The use of freeze-dried biomass for metabolite extraction and data normalisation was more efficient at measuring the active state of the intracellular metabolites in M. alpina, and an important role of amino acid oxidation in the nitrogen limitation-mediated lipid accumulation was found.

TL;DR: The objective of the present study was to reveal the effects of four types of nitrogen source on the lipid metabolism of the oleaginous fungus M. alpina using untargeted lipidomics, targeted fatty acid and RT-qPCR analysis, and the importance of an optimal nitrogen source for ARA-rich oil production.

TL;DR: In this paper, the authors provide an overview of the major research methods, key factors governing lipid biosynthesis, metabolic engineering and omics studies in the oleaginous fungus Mortierella alpina.

TL;DR: This study found that overexpression of homologous AMPD in Mortierella alpina favoured lipid synthesis over cell growth, and coupling energy balance with lipogenesis provided new insights into cell metabolism under nitrogen-limited conditions and targets to regulate fatty acids accumulation.