Showing papers by "Henry A. Erlich published in 1981"

Method for producing an analytical antibody probe, an analytical antibody probe, and a method for analysing a sample for certain antibodies

Abstract: The complexity of antigenic test material in tests which employ the antigen-antibody reaction increases the likelihood of false results. In particular, the complexity of antigenic test material in diagnostic techniquest increases the likelihood of false positive signals generated due to antibodies stimulated by organisms showing some but all antigenic properties with the test material. In conventional tests, a single positive or negative signal is obtained which is the result of a complex and undifferentiated array of structural components of an organism or material antigenically related to it. In contrast, the present invention greatly expands the use of the antigen-antibody reaction in analysis techniquest and diagnosis by providing a multi-signal. The invention provides a method for producing an analytical antibody probe for detecting the presence of antibodies specific for particular protein and/or poly- I saccharide material, which method comprises disposing I upon a solid-state substrate in a pre-determined spatial relationship a set of differentiated antigenic components which have been derived from said protein or polysaccharide material, said set of differentiated antigenic components being selected such that at least a subset thereof is known to be reactive with said antibodies. The invention also embraces the analytical antibody probe as such and a corresponding method for analysing a sample to determine the presence and distribution of antibodies specific for particular protein and/or polysaccharide material.

Diagnostic method and probe

Abstract: Method is described for diagnosing for the presence of a specific desease, a specific stage of a disease, or an allergy in a patient. Differentiated antigenic components of a larger protein or polysaccharide group are supported in spaced relation on a solid-state substrate. At least a subset of the components is known to be reactive with antibodies present in serum obtained from a patient having the specific disease, a specific stage of a disease, or an allergy. Upon exposure to the patient's serum, antigen-antibody reactions on the substrate are detected for correspondence with a known pattern specific for the disease, the specific stage of the disease, or the allergy. Also described is a method for producing such a diagnostic antibody probe and the probe itself.