Nanotechnologies for the delivery of biologicals: Historical perspective and current landscape.

We have been investigating the potential use of polymers modified with cell-penetrating peptides as an adjuvant for mucosal vaccination and have already developed nondegradable poly(N-vinylacetamid...

Biodegradable Hyaluronic Acid Modified with Tetraglycine-l-octaarginine as a Safe Adjuvant for Mucosal Vaccination.

Nasal absorption enhancement of protein drugs independent to their chemical properties in the presence of hyaluronic acid modified with tetraglycine-L-octaarginine

The detection of hydrogen peroxide (H2O2) is of great significance in environmental monitoring, enzymatic reactions, and disease diagnosis. Here we present the peptide-mediated biomimetic synthesis of ultrafine platinum nanowires (PtNWs) on graphene oxide (GO) nanosheets for the formation of functional hybrids, which show high potential for the fabrication of colorimetric and electrochemical sensors for the detection of H2O2 with high performance. A multifunctional peptide with the sequence KIIIIKYWYAF was designed to create peptide nanofibers (PNFs) via a controllable self-assembly process, which serves as a bridge between GO nanosheets and PtNWs to form PtNWs-PNFs/GO hybrids. On this basis, a dual-mode sensor platform for both colorimetric and electrochemical sensing of H2O2 was fabricated successfully. The obtained results indicate that the synthesized PtNWs-PNFs/GO hybrids could catalyze the decomposition of H2O2 to generate ˙OH radicals with a significant current response, and the ˙OH radicals are capable of overoxidizing 3,3',5,5',-tetramethylbenzidine (TMB), producing a blue-colored species with a distinct color change for colorimetric sensing. In addition, due to its high catalytic activity, the fabricated PtNWs-PNFs/GO hybrid-based electrochemical sensor exhibits a wider linear detection range of 0.05 μM-15 mM and a low detection limit of 0.0206 μM, which can be applied to detect H2O2 with high selectivity and sensitivity. Our study provides a green and environmentally friendly synthetic strategy for the preparation of biomimetic materials from PtNWs, and the fabricated colorimetric/electrochemical dual-mode H2O2 sensor platform will have a great impact in bioanalysis, environmental monitoring, and biomedicine.

Biomimetic graphene-supported ultrafine platinum nanowires for colorimetric and electrochemical detection of hydrogen peroxide.

Acquisition of Absorption-enhancing Abilities of Cationic Oligopeptides with Short Chain Arginine Residues through Conjugation to Hyaluronic Acid.

Biocompatible Polymers Modified with d-Octaarginine as an Absorption Enhancer for Nasal Peptide Delivery.

The present invention provides a composition with weak cytotoxicity for introducing a nucleic acid, such as a short oligonucleotide or a gene, into a cell and expression of the gene in the cell with improved capability for introduction of the nucleic acid, and a novel compound contained in the composition. When a compound represented by a formula (I): 
        (R 1 )n -R 2 -R 3      (I) 
wherein (R 1 )n represents a polyamino acid residue consisting of a total of n amino acid residues, which are identical to or different from one another, the n residues being selected from the group consisting of an arginine residue, a lysine residue, and a histidine residue, and n being an integer of from 4 to 16; R 2 represents a single bond or an amino acid residue; and R 3 represents a phospholipid residue with 1 or 2 identical or different unsaturated fatty acid residues having from 12 to 20 carbon atoms, and a lipid are administered or supplied to a cell together with a nucleic acid such as a gene, the nucleic acid can be efficiently introduced with weak cytotoxicity.

Hideo Kobayashi

Papers

Biocompatible Polymers Modified with d-Octaarginine as an Absorption Enhancer for Nasal Peptide Delivery.

Nasal absorption enhancement of protein drugs independent to their chemical properties in the presence of hyaluronic acid modified with tetraglycine-L-octaarginine

Composition for introduction of nucleic acid