I
I. Barker
Researcher at Central Science Laboratory
Publications - 40
Citations - 2540
I. Barker is an academic researcher from Central Science Laboratory. The author has contributed to research in topics: Potato virus Y & Plant virus. The author has an hindex of 26, co-authored 40 publications receiving 2408 citations.
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Journal ArticleDOI
Detection of Potato mop top virus and Tobacco rattle virus Using a Multiplex Real-Time Fluorescent Reverse-Transcription Polymerase Chain Reaction Assay.
TL;DR: The development of a multiplex assay for the detection of TRV and PMTV directly from potato tubers and leaves by polymerase chain reaction (PCR) combined with in-tube fluorescent product detection (TaqMan) obviates any post-PCR manipulations and has many advantages including reducing contamination risks, eliminating the need for ethidium bromide staining, and removing the time and cost of gel running.
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The detection of Tomato spotted wilt virus (TSWV) in individual thrips using real time fluorescent RT-PCR (TaqMan)
TL;DR: The development of a sensitive and robust, high-throughput method for the detection of TSWV in individual insects based on TaqMan chemistry, which incorporates a novel RNA specific internal control to increase the reliability of the results.
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Development of a real-time RT-PCR assay for the detection of Potato spindle tuber viroid.
Neil Boonham,L González Pérez,Marcelo Méndez,E Lilia Peralta,A. Blockley,K. Walsh,I. Barker,Rick Mumford +7 more
TL;DR: A one-tube real-time RT-PCR assay based on TaqMan chemistry was developed and was shown to be able to detect a wide range of isolates of PSTVd and in comparison with a chemi-luminescent hybridisation system was shows to be 1000-fold more sensitive.
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Faster, simpler, more-specific methods for improved molecular detection of Phytophthora ramorum in the field.
TL;DR: A scorpion real-time PCR assay that is twice as fast as TaqMan was developed, allowing the detection of P. ramorum in less than 30 min and a loop-mediated isothermal amplification assay, which allowed sensitive and specific detection of the pathogen.
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On-site DNA extraction and real-time PCR for detection of Phytophthora ramorum in the field.
TL;DR: This is the first description of a method for DNA extraction and molecular testing for a plant pathogen carried out entirely in the field, independent of any laboratory facilities.