Micellar solutions as reaction media

Reversed micelle solvents as tools of enzyme purification and enzyme-catalyzed conversion.

Amino acid extraction and mass transfer rate in the reverse micelle system

In this work the possibility of integrating the two process steps—extraction of an enzyme from its source aqueous solution into an organic solvent containing reversed micelles and its stripping into another aqueous medium of appropriate composition is studied. The kinetics of enzyme mass transfer during the integrated process is investigated using a stirred cell with two separated compartments. The enzyme/surfactant system chosen consisted of α-amylase and the cationic surfactant CTAB (cetyl trimethyl ammonium bromide). Comparison between the classical extraction process and the “integrated reversed micellar extraction–stripping” is made. The enzyme activity changes during the transfer process are followed, and the registered tendencies are discussed. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 583–592, 1999.

Integrated reversed micellar extraction and stripping of ?-amylase

Reaction kinetics in reactive extraction for chiral separation of α-cyclohexyl-mandelic acid enantiomers with hydroxypropyl-β-cyclodextrin

Mass transfer characterization in forward and back extractions of lysozyme by AOT-isooctane reverse micelles across a flat liquid-liquid interface

Nishiki, T, Sato, I and Kataoka, T, 1990 Separation and concentration of proteins with liquid-liquid extraction using reversed micelles Protein extractions using Aerosol OT (AOT)-isooctane reversed micelle solutions have been studied to explore the potential for separating and enriching proteins with the reversed micellar extraction The effects of pH, ionic strength and chlorides in a bulk aqueous phase, and AOT concentration in an organic phase on the partitioning of lysozyme and myoglobin and the solubilization of water were examined in detail Lysozyme could be enriched into the reversed micellar phase up to thirty times the aqueous feed concentration Lysozyme in the reversed micellar phase was efficiently stripped with potassium chloride solutions, whose concentration was 1-2 kmol/m 3 and pH was 11-12 The results indicated that the separation of two proteins and the concentration of lysozyme are capable

I. Sato

Papers

Mass transfer characterization in forward and back extractions of lysozyme by AOT-isooctane reverse micelles across a flat liquid-liquid interface

Separation and Concentration of Proteins with Liquid-Liquid Extraction Using Reversed Micelles