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Ilya V. Manukhov

Researcher at Moscow Institute of Physics and Technology

Publications -  64
Citations -  629

Ilya V. Manukhov is an academic researcher from Moscow Institute of Physics and Technology. The author has contributed to research in topics: Escherichia coli & Medicine. The author has an hindex of 12, co-authored 51 publications receiving 454 citations. Previous affiliations of Ilya V. Manukhov include Kurchatov Institute & National Research University – Higher School of Economics.

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A gene encoding L-methionine γ-lyase is present in Enterobacteriaceae family genomes : identification and characterization of Citrobacter freundii L-methionine γ-lyase

TL;DR: The nucleotide sequence of the hybrid plasmid with a C. freundii EcoRI insert of about 3.0 kbp contained two open reading frames, consisting of 1,194 and 1,296 nucleotides, respectively as mentioned in this paper.
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Lux-biosensors for detection of SOS-response, heat shock, and oxidative stress

TL;DR: It was shown that the SOS-biosensor pColD is superior in main characteristics to the earlier constructed biosensor pRecA, and the heat biosensors pIbpA is more efficient than theEarlier constructed pGrpE.
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Action of 1,1-dimethylhydrazine on bacterial cells is determined by hydrogen peroxide.

TL;DR: Seven different recombinant bioluminescent strains of Escherichia coli containing the promoters katG and soxS, responsive to oxidative damage, and lac, fused to the bacterial operon from Photorhabdus luminescens, were used to describe the mechanism of toxicity of 1,1-dimethylhydrazine on bacteria, as well as to determine whether bacteria can sensitively detect the presence of this compound.
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L-methionine γ-lyase from Citrobacter freundii: Cloning of the gene and kinetic parameters of the enzyme

TL;DR: It is demonstrated that a presence of a hybrid plasmid pUCmgl, containing the megL gene in the E. coli K12 cells, leads to a decrease in efficiency of EcoKI-restriction.
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Comparative Analysis of the lux Operons in Aliivibrio logei KCh1 (a Kamchatka Isolate) and Aliivibrio salmonicida

TL;DR: A new psychrophilic strain, KCh11, of marine luminescent bacteria classified as Aliivibrio logei is provided, and it was demonstrated that the reduced production of bioluminescence in A. salmonicida is most likely defined by a specific defect in its luxD gene.