SUMMARYCSF protein electrophoresis in agar gel was performed in 2,043 patients. Among them, 323 patients were classified as having MS. The essential feature of the gamma globulins in CSF from MS patients and other inflammatory CNS diseases resided in their restricted heterogeneity distribution. This peculiar electrophoretic pattern occurs in 75.2 to 86.9% of MS patients, depending upon the diagnostic category (possible, probable, or definite). The differences were significant. In MS patients there was also a positive correlation between the degree of disability and the frequency of gamma pattern when Grade I and Grade III were compared, but no correlation was found between the duration and course of the disease on the one hand and the CSF gamma globulin changes on the other hand. In the other patients, this frequency varied between 2. to 5.1%, except for the group with inflammatory CNS diseases in which it reached 39.6%. The diagnostic value of the method employed is discussed and emphasized.

https://n.neurology.org/content/neurology/20/10/982.full.pdf

Electrophoretic morphology of gamma globulins in cerebrospinal fluid of multiple sclerosis and other diseases of the nervous system

Free amino acids and peptides are markedly hydrophilic compounds, only slightly soluble in non-aqueous solvents. This must be borne in mind when sampling and preparing materials for TLC as well as when choosing the solvent. Some data on solubilities of a few amino acids in various solvents which illustrate this are:

Amino Acids and Derivatives

One-Phase Solvent Mixtures for Separation of Amino Acids

The selective degradation of injected proteins occurs principally in the cytosol rather than in lysosomes

Clinical data and the identification of special forms of multiple sclerosis in 1271 cases studied with a standardized documentation system.

Circular Paper Chromatography

http://science.sciencemag.org/content/sci/119/3082/124.2.full.pdf

Circular paper chromatography. II. Isatin as a color reagent for amino acids.

Color Reaction oK Amino Acids with Alloxan, Isatin, and Ninhydrin in Circular Paper Chromatography

IN PREVIOUS studies* a characteristic pattern of protein changes was observed both in the blood serum and in the cerebrospinal fluid in about 80% of the patients with typical multiple sclerosis. The changes in these biological fluids examined simultaneously were termed the "protein profile." The variations in serum proteins, as determined electrophoretically, 1 consisted of significantly decreased albumin and A/G ratio values, markedly increased alpha-2 and beta globulin fractions, a slightly elevated (or normal) gamma globulin fraction, and a normal alpha-1 globulin fraction. Similarly, the cerebrospinal fluid proteins in the same patients showed markedly elevated total protein and gamma globulin values, as well as increased gamma globulin-total protein (G. G./T. P.) ratios. The present study was undertaken to enlarge our case material for purposes of statistical analysis and to investigate changes of the "protein profile" in comparison with the clinical findings, over extended periods of time. Furthermore, the use of

https://jamanetwork.com/HttpHandlers/ArticlePdfHandler.ashx?journal=NEURPSYCH&articleId=651915&pdfFileName=archneurpsyc_73_1_009.pdf

Irwin Oreskes

Papers

Circular Paper Chromatography

Circular paper chromatography. II. Isatin as a color reagent for amino acids.

Color Reaction oK Amino Acids with Alloxan, Isatin, and Ninhydrin in Circular Paper Chromatography

Protein profile in multiple sclerosis.

Qualitative determination of amino acids in protein hydrolyzates by circular paper chromatography.