Ivica Králová-Hromadová

TL;DR: Short (<500 bp) variable regions suitable for broader biogeographical studies of giant liver fluke were designed and determined to allow molecular differentiation of F. magna and F. hepatica.

TL;DR: Evaluating the utility of two nuclear and two mitochondrial molecular markers for use in circumscribing generic boundaries and estimating interrelationships in Caryophyllidean cestodes shows that these commonly employed markers do not contain sufficient signal to infer well-supported phylogenetic estimates due to substitution saturation.

TL;DR: Haplotypes found in Italy were distinct from those determined in the remaining European localities which indicates that introduction of F. magna to the European continent occurred more than once, and in the Czech focus of infection, a south-eastern USA origin was revealed.

TL;DR: The molecular analysis of all seven valid species of Khawia, based on comparison of sequences of two nuclear ribosomal and two mitochondrial genes, has shown that the species form three major groups clustered according to their fish hosts.

TL;DR: Application of the primers is of particular value for molecular differentiation of morphologically hardly distinguishable F. hepatica, F. magna and P. cervi eggs after coprological examinations.