Check List of the Helminth Parasites of African Mammals of the Orders Carnivora, Tubulidentata, Proboscidea, Hyracoidea, Artiodactyla and Perissodactyla.

Monitoring of gastrointestinal nematode parasites in ruminants (domestic and wild) is often based on fecal examination techniques, looking for excreted eggs and larval forms using morphological keys. These, are more available in domestic ruminants, in which helminths are widely studied, than in wild ruminants.  This study tried to provide certain morphological elements that will help to recognize the L3 larvae of Camelostrongylus mentulatus and Nematodirus spathiger that could parasite either domestic or wild ruminants. For that, we resorted first to the culture of L3 larvae from fecal samples taken from African antelopes, and second by the microscopic characterization of each isolated larval morphological pattern previously identified by sequencing of its internal transcribed spacer (ITS-2) regions of the ribosomal DNA. The results of different microscopic captured images showed that Camelostrongylus mentulatus larva is 16 intestinal cells that measuring approximately 820 µm length, ‎≈ 25 µm wide, and ‎≈ 47 µm for its sheath tail extension and by this be closer to Teladorsagia circumcincta characteristics.  For Nematodirus spathiger, it possesses 8 gut cells and measuring about 1020 µm long, ‎≈ 25 µm wide, and‎ ≈ 143 µm for its sheath tail extension with specific tail appendages. Have done this, we were able to get some clarifications on the morphology of the studied larvae, and we believe thus that this study will contribute to the establishment of morphological identification keys especially for parasitic nematodes of wild ruminants.

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Some larval morphological characteristics of Camelostrongylus mentulatus and Nematodirus spathiger

Abstract: Trichuris spp. are nematode parasites infecting many species, including domestic and wild ruminants in zoological and wildlife parks worldwide. These nematodes cause significant morbidity in giraffes (Giraffa camelopardalis) and other hoofstock. Parasite transmission between ruminant species is well reported; however, relative to domestic species, little is known about Trichuris infections in giraffes under human care. We hypothesized that Trichuris spp. differ between individual giraffes in different US regions, suggesting giraffes are susceptible to Trichuris from other ruminant hosts. The study sites used to assess this hypothesis included The Wilds in Cumberland, Ohio; Fossil Rim Wildlife Center in Glen Rose, Texas; White Oak Conservation in Yulee, Florida; and Binder Park Zoo in Battle Creek, Michigan. Trichuris eggs were collected from the feces of 14 individual giraffes located at the four different study sites and from soil samples from the enclosures where Trichuris-positive giraffes were housed. The eggs were isolated and their genes were amplified by PCR and compared at the molecular level. Trichuris samples from four giraffe hosts and one soil site were sequenced and portions of the cox1 and 18S genes compared. This study found that >12 eggs per gram of fecal-derived Trichuris eggs must be present to amplify Trichuris-specific DNA. The Trichuris spp. found in the majority of giraffes in this study were most similar to T. ovis and T. discolor, and one giraffe sample had greater similarity to T. skrjabini and T. leporis.

Detection and differentiation of trichuris in giraffe (giraffa camelopardalis) under human care

Trichuris spp. are nematode parasites infecting wild ruminants in zoological institutions worldwide. These helminths cause significant morbidity in giraffe (Giraffa camelopardalis) and other hoofstock located in zoological institutions throughout the United States. Historically, studies and institutions have used a variety of nematode detection methods with various flotation solutions. Optimization of Trichuris egg detection is necessary for monitoring collections. Fecal and soil optimized protocols were generated in this study using samples containing Trichuris eggs from multiple semi free-ranging zoological institutions. First, Sheather's sugar (specific gravity (SG) 1.27), sucrose (SG 1.40), magnesium sulfate (SG 1.26), and zinc sulfate (SG 1.18) were compared as flotation solutions by quantitative eggs per gram using a modified Stoll method. Then a soil recovery method was optimized comparing Tween 20, sodium hydroxide, Dawn™ (Procter and Gamble) detergent, and sodium chloride as liberating solutions to free eggs from the soil. We found that Sheather's sugar and sucrose solutions were the most effective for Trichuris egg detection, and either sodium hydroxide or sodium chloride liberated eggs from soil.

Detection of Trichuris eggs in feces and soil from giraffe (Giraffa camelopardalis) and other hoofstock enclosures under human care in the USA

Parasite prevalence was investigated in giraffes (Giraffa camelopardalis) housed in six major Czech zoological gardens: Zoo Ostrava, Zoo Dvůr Kralove nad Labem, Zoo Liberec, Zoo Olomouc, Zoo Praha, and Zoo Plzeň. In autumn 2012 and in spring 2013, 120 faecal samples from 21 animals were examined using the McMaster egg counting technique. Propagative stages of three parasite groups were discovered, namely eggs of the nematodes of the order Strongylida (prevalence 25.8%), whipworms Trichuris spp. (prevalence 25%), and oocysts of the unicellular coccidia of the genus Eimeria (prevalence 1.7%). The results indicate that captive giraffes in the Czech zoos are not substantially affected by parasitic infection.

https://sciendo.com/pdf/10.1515/sab-2017-0019

J. Drnek

Papers

Gastrointestinal Parasites in Giraffes Kept in Zoological Gardens of the Czech Republic