In this research, silver‐doped zinc oxide (SdZnO) nanoparticles (NPs) were synthesized in an environmental‐friendly manner. The synthesized NPs were identified by UV–vis spectroscopy, X‐ray diffraction (XRD), and scanning electron microscopy (SEM). Finally, the antimicrobial activity of synthesized ZnO and SdZnO NPs was performed. It was observed that by doping silver, the size of ZnO NPs was changed. By adding silver to ZnO NPs, the antimicrobial effect of ZnO NPs was improved. Antibacterial test against gram‐positive bacterium Streptococcus mutants showed that SdZnO NPs with a low density of silver had higher antibacterial activity than ZnO NPs; Therefore, SdZnO NPs can be used as a new antibacterial agent in medical applications.

Plant‐mediated synthesis of silver‐doped zinc oxide nanoparticles and evaluation of their antimicrobial activity against bacteria cause tooth decay

Multifaceted role of polyphenols in the treatment and management of neurodegenerative diseases.

Abstract Developing biosynthesis of silver nanoparticles (Ag‐NPs) using plant extract is an environmentally friendly method to reduce the use of harmful chemical substances. The green synthesis of Ag‐NPs by Lawsonia inermis extract and its cellular toxicity and the antimicrobial effect was studied. The physical and chemical properties of synthesised Ag‐NPs were investigated using UV‐visible spectroscopy, infrared spectroscopy, X‐ray diffraction (XRD), scanning, and transmission electron microscopy. The average size of Ag‐NPs was 40 nm. The XRD result shows peaks at 2θ = 38.07°, 44.26°, 64.43°, and 77.35° are related to the FCC structure of Ag‐NPs. Cytotoxicity of synthesised nanoparticles was evaluated by MTT toxicity test on breast cancer MCF7 cell line. Observations showed that the effect of cytotoxicity of nanoparticles on the studied cell line depended on concentration and time. The obtained IC50 was considered for cells at a dose of 250 μg/ml. Growth and survival rates decreased exponentially with the dose. Antimicrobial properties of Ag‐NPs synthesised with extract were investigated against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus to calculate the minimum inhibitory concentration and the minimum bactericidal concentration of (MBC). The results showed that the synthesised Ag‐NPs and the plant extract have antimicrobial properties. The lowest concentration of Ag‐NPs that can inhibit the growth of bacterial strains was 25 μg/ml.

Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application

Oxidative Stress and Its Effects on Male Infertility: A Review Study

Research on multi-target tracking technology based on machine vision

Physical and chemical changes caused by oxidative stress in the spermatozoa membrane can reduce spermatozoa function and even lead to death. Cystamine (NH2-CH2-CH2-SH, β-mercaptoethylamine) is a natural substance that modulates the endocrine and metabolic status of animals. This substance has antioxidant and anti-apoptotic effects by inducing intracellular cysteine accumulation. Cystamine is used to treat many diseases despite its many side effects. Sheep semen is sensitive to the stressful condition of chilling storage, which restricts semen storage for artificial insemination in commercial herds. The effect of cystamine on spermatogenesis is not yet fully understood. The present study aimed to investigate the effect of cysteamine addition to the sheep sperm extender during cooling storage on semen quality parameters. Sperm samples were collected from six Edilbayevskaya rams (2 and 3 years old, 70-85 kg). The samples were diluted by extender and supplemented with different concentrations of cysteamine (0, 1, 2, 5, and 10 mM) and cooled to 4oC for 50 h. Motility parameters, membrane integrity, viability, lipid peroxidation, and mitochondrial activity of cooled semen were evaluated at 0, 25, and 50 h of cooling storage. Although cysteamine failed to affect semen quality at start time (0 hrs), extender supplementation with cysteamine improved sperm total motility, progressive motility, and mitochondrial membrane potential during storage periods (P≤0.01). Moreover, using 1 and 2 mM cysteamine functionally and viably improved (P≤0.01) sperm membrane compared to other treatments. Antioxidant potential (AOP), lipid peroxidation (LPO), and total glutathione (tGSH) (except AOP at 50 h) were significantly different after semen storage at 4 °C. Therefore, levels of AOP and tGSH were significantly increased by using cysteamine. Cysteamine supplementation (1 and 2 mM cysteamine) leads to lower levels of LPO (p<0.01) at 0, 25, and 50 h. Therefore, finding and using the best concentrations of cysteamine in a cooling extender could be effective in saving sheep semen against damages of the cooling storage process.

J Jumintono

Papers

Effect of Cystamine on Sperm and Antioxidant Parameters of Ram Semen Stored at 4 °C for 50 Hours