A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.

Publisher Summary This chapter discusses the procedures employed for the analysis of the sugar components of glycoproteins. Most of the methods used for the estimation of the monosaccharides present in glycoproteins are colorimetric in nature. Because of the relatively low specificity of some of these color reactions, it is often important to apply them only after appropriate separation of the constituents. In many cases complete identification, preferably by chromatographic means, of all the sugar and amino acid constituents of a glycoprotein should precede any colorimetric analysis so that potentially interfering substances may be taken into account. The hydrolytic release of the monosaccharides is also considered in the chapter. The optimal conditions of acid hydrolysis for the monosaccharides vary substantially because of differences both in the stability of their glycosidic bonds and in their susceptibility to destruction during the hydrolysis. One of the prerequisites for an accurate analysis of the monosaccharide components of a polymer is finding hydrolysis conditions under which all of a given sugar is released and yet is not significantly destroyed.

[1] Analysis of sugars found in glycoproteins

A rapid filter paper assay for UDPglucose-glycogen glucosyltransferase, including an improved biosynthesis of UDP-14C-glucose.

Peroxidase Isozymes from Horseradish Roots I. ISOLATION AND PHYSICAL PROPERTIES

Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes.

THE ammoniacal silver nitrate spray1 used for the detection of sugars has several disadvantages; to those mentioned by Partridge2 should be added the necessity for very careful control of the heating step, particularly important in laboratories lacking special apparatus. We have introduced modifications, based on a test given by Feigl3 for reducing sugars, which eliminate the heating step, and in which the reagents are applied in organic solvents, thus removing the danger of migration of the sugar spots. The method has been in use for more than a year, and has proved easy to handle and extremely reliable.

J S Harrison

Papers

Detection of sugars on paper chromatograms.