Regulation of trehalose mobilization in fungi.

Publisher Summary Recent monumental advances in microbial genetics and in the chemistry of macromolecular biosynthesis have been applied to problems with developmental import The program's appearance and macromolecules' disappearance plays crucial roles in developmental sequences As a consequence, a new technology has arisen that must be adapted to the peculiarities of each kind of biological material This chapter describes genetic, biochemical, and immunochemical techniques that have been successfully applied in the laboratory to the study of cellular slime mold development The cellular slime molds are designated as an order within the phylum Myxomycophyta Several genera and many species have been described However, the bulk of the current investigation has been carried out with Dictyostelium discoideum  This species has a particularly interesting morphogenetic sequence and constructs fruiting bodies rapidly and well under a wide variety of environmental conditions Many mutant strains have been isolated that display a wealth of developmental aberrations

Chapter 14 Biochemical and Genetic Methods in the Study of Cellular Slime Mold Development

Chemical dissolution and in vitro reconstruction of sponge cell adhesions. i. isolation and functional demonstration of the components involved.

The intracellular turnover of protein and nucleic acids and its role in biochemical differentiation

1 0 3 (2) Electrostatic attractive forces (3) London-van der Waals forces (4) The interaction of London and electrostatic forces . ( 5 ) Other factors . . . IX. The specificity of adhesion . . (I) Antigen-antibody type binding . . . . (2) Specific patterning . . X. Non-specific adhesion . . . (I) Binding by intercellular material . . . . (2) Other effects of intercellular material . . . . XI. The dynamic nature of the interaction of cells . . . XII. Summary . . . . . XIII. References . . . . . XIV. Addendum . . . . .

Cell contact and adhesion.

Morphogenesis and ultrastructure inDictyostelium

1. Antibodies to slime molds were produced by injecting D. discoideum and D. purpureum amebas from 48 hour cultures into rabbits. 2. Anti-D. discoideum and anti-D. purpureum sera caused agglutination of homologous amebas from 24 to 26 hour cultures, agglutination of certain heterologous amebas from 30 to 36 hour cultures, and agglutination of all heterologous amebas from 43 to 48 hour cultures. 3. The data show that new surface antigens are formed in cultures after 26 hours and it is suggested that the new antigens are concerned with cell adhesion. 4. The probable role of surface antigens in the interaction of cells of different species of slime molds was discussed.

/pdf/serological-investigations-of-cell-adhesion-in-the-slime-3klq8aa7gb.pdf

James H. Gregg

Papers

Morphogenesis and ultrastructure inDictyostelium

Serological investigations of cell adhesion in the slime molds, Dictyostelium discoideum, Dictyostelium purpureum, and Polysphondylium violaceum.

Patterns of cell differentiation revealed by l-[3H]fucose incorporation in Dictyostelium

Regulation in the cellular slime molds.

Unit membrane structural changes following cell association in Dictyostelium.