Janice Lau

TL;DR: The heterologous production of epothilone D in Myxococcus xanthus was improved by 140-fold with the incorporation of an adsorber resin, the identification of a suitable carbon source, and the implementation of a fed-batch process, demonstrating the significant role of metal ions in cell metabolism and in epothILone biosynthesis.

TL;DR: Resorcylic acid lactones having a C5-C6 cis double bond and a ketone at C7 and other compounds capable of Michael adduct formation are potent and stable inhibitors of a subset of protein kinases having a specific cysteine residue in the ATP binding site.

TL;DR: Through optimization of the medium composition and fermentation conditions, the maximum cell density was increased by two-fold, and a final titer of 1.1 g l(-1) of 6-dEB was achieved, an 11-fold improvement compared to the highest reported titer with E. coli as the production host.

TL;DR: This is the first reported crystallization of 1 from a binary solvent system that consisted of ethanol and water, and the product was recovered as white crystals with a final purity of > or =97% (w/w).

TL;DR: It is found that at least three other epothilones can also be produced as the major fermentation product of this recombinant strain by supplementation of specific substrates to the production media.