Jason C. Goodrick

TL;DR: Continuous production of the enzyme by P. pastoris with the GAP promoter was demonstrated in a 1.5-L working volume fermentor using either glucose or glycerol as the carbon source and no proteolytic degradation was seen in the continuous fermentation mode.

TL;DR: The feasibility of large‐scale production of recombinant human chitinase using a constitutive Pichia pastoris expression system was demonstrated in a 21‐L continuous stirred tank reactor and all large-scale cultivations were conducted without molecular oxygen supplementation.

TL;DR: A continuous fermentation process has been developed in Pichia pastoris (P. pastoris) is order to produce large quantities of recombinant human proteins as mentioned in this paper, which can be extended for long periods of time with an excellent steadystate protein concentration and cell densities achieved.

TL;DR: A serum-free eukaryotic cell culture medium supplement consisting of carbon sources, vitamins, inorganic salts, amino acids and a protein digest has been proposed in this paper to enable the maintenance of mammalian cell cultures at cell densities equal to or greater than that obtained with batch culture methods.

TL;DR: In this article, Pichia Pastoris (P.pastoris) introduced a process of fermentation continué, which is based on un procede de fermentation continu qui a ete mis au point dans la levure pichia pastoris, in order to produce quantites elevees de proteines humaines recombinees.