Mapping the extended substrate binding site of cathepsin G and human leukocyte elastase. Studies with peptide substrates related to the alpha 1-protease inhibitor reactive site.

Doxorubicin-loaded poly(ethylene glycol)–poly(β-benzyl-l-aspartate) copolymer micelles: their pharmaceutical characteristics and biological significance

Comparisons of Indefinite Self-Association Models

An exact knowledge of the structure, dynamics, and reactions of molecules provides the key to understand their functions and properties. NMR spectroscopy has developed, through the introduction of two-dimensional methods, into the most important method for the investigation of these questions in solution. A great variety of different techniques is available. However, for their successful application not only the appropriate equipment is required, but also the right choice of experiments and optimum measurement parameters, as well as a careful evaluation of the spectra. This contribution describes the necessary background for modern NMR spectroscopy. With the aid of the so-called product operator formalism it is possible to understand pulsed Fourier transform NMR spectroscopy both qualitatively and quantitatively. Very few, readily understandable assumptions are sufficient for confident application of these methods. This article attempts to introduce in a simple manner this formalism as well as phase cycles necessary for the understanding of pulse sequences, and to train the reader through the discussion of several 2D NMR techniques. An overview of the most important techniques is given in the second part of this article.

Two‐Dimensional NMR Spectroscopy: Background and Overview of the Experiments [New Analytical Methods (36)]

Inhibition of the serine proteases leukocyte elastase, pancreatic elastase, cathepsin G, and chymotrypsin by peptide boronic acids.

https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2872%2980529-5

Interaction of oligopeptides containing aromatic amino acids with nucleic acids. Fluorescence and proton magnetic resonance studies

https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2874%2980322-4

Self-association of daunorubicin.

Interactions of tyrosine and tyramine with nucleic acids and their components. Fluorescence, nuclear magnetic resonance and circular dichroism studies.

A double-delay SR-MGE-SNAP sequence allowing simultaneous T1 and T2* measurement was developed for integrating arterial input function (AIF) measurement into DCE MRI. Implemented on a 4.7-T animal MR system, this technique was applied to mice with colorectal tumor xenografts. AIF, measured in the mouse heart, was modeled by a bi-exponential function, whereas tumor K(trans) and v(e) parameter maps were obtained from analysis with a two- compartment model using an individually measured AIF. AIF analysis of T2*-corrected data yielded A1 = 9.2 +/- 4.3 kg/l, A(2) = 4.2 +/- 0.8 kg/l, m1 = 2.3 +/- 1.1 min(-1), and m2 = 0.05 +/- 0.02 min(-1). The mean initial plasma concentration C ( p )(t = 0) = 8.0 +/- 2.7 mM was compatible with estimated 8.6 mM. Without T2*-correction distribution phase parameters A1, m1, and C(p)(t = 0) were underestimated. In tumors, neglect of T2* effects yielded mean K(trans) values which were reduced by 14% (P < 0.05), whereas v(e) showed only a slight non-significant reduction. Simultaneous measurement of DeltaR1 and DeltaR2* studied in highly and poorly vascularized and (pre-)necrotic tumor regions revealed complementary behavior of both parameters with respect to vascular properties. In conclusion, the presented measurement technique is a promising tool for dynamic MRI applications studied in animal models at high field strengths and/or with CA of high relaxivities, as it combines classical DCE MRI integrating AIF assessment with dynamic T2* measurement.

Simultaneous dynamic T1 and T2* measurement for AIF assessment combined with DCE MRI in a mouse tumor model.

Interactions entre acides aminés et acides nucléiques. III — Etude par absorption et résonance magnétique nucléaire de la formation de complexes entre le tryptophane et les constituants des acides nucléiques

Jean-Luc Dimicoli

Papers

Interaction of oligopeptides containing aromatic amino acids with nucleic acids. Fluorescence and proton magnetic resonance studies

Self-association of daunorubicin.

Interactions of tyrosine and tyramine with nucleic acids and their components. Fluorescence, nuclear magnetic resonance and circular dichroism studies.

Simultaneous dynamic T1 and T2* measurement for AIF assessment combined with DCE MRI in a mouse tumor model.

Interactions entre acides aminés et acides nucléiques. III — Etude par absorption et résonance magnétique nucléaire de la formation de complexes entre le tryptophane et les constituants des acides nucléiques