Showing papers by "Jeffrey L. Evelhoch published in 1995"
TL;DR: In vivo 31P NMR measurements of normal mouse liver indicate that isoflurane has a similar effect in the liver and suggests that a pool of P1 in a lower pH environment may become evident under isofLurane anesthesia.
Abstract: The principal aim of these studies was to evaluate the utility of isoflurane and halothane for NMR investigations of tumor physiology. In vivo 31 P and 2 H NMR were used to examine RIF-1 tumors before, during, and (for 31 P) after anesthesia. In tumors, halothane decreases blood flow, [PCR]:[NTP], and pH indicated by the P i chemical shift (pH nmr ), while it increases [P i ]:[NTP]; effects consistent with well-established cardiovascular effects of halothane. Isoflurane does not affect tumor blood flow or [PCr]:[NTP], but increases tumor [P i ]:[NTP] and decreases tumor pH nmr . In vivo 31 P NMR measurements of normal mouse liver (upper abdomen) indicate that isoflurane has a similar effect in the liver. Although the mechanism for these effects is unknown, observation of a split P i peak during isoflurane anesthesia suggests that a pool of P i in a lower pH environment may become evident under isoflurane anesthesia. Regardless of the cause for increased [P i ]:[NTP] and decreased pH nmr , the utility of isoflurane anesthesia for 31 P NMR studies of energy metabolism is limited
15 citations